Ghlissi Zohra, Hakim Ahmed, Mnif Hela, Zeghal Khaled, Rebai Tarek, Boudawara Tahiya, Sahnoun Zouheir
Research Unit of Pharmacology and Toxicology of Xenobiotics (UR12 ES13), Faculty of Medicine, University of Sfax, 3029, Sfax, Tunisia.
Anatomopathology Laboratory, CHU Habib Bourguiba, University Hospital, Sfax, Tunisia.
Saudi J Kidney Dis Transpl. 2018 May-Jun;29(3):545-553. doi: 10.4103/1319-2442.235168.
This study was performed to investigate the protective effect of combined use of Vitamins E and C on colistin-induced tubular damage in rat. Animals were treated with sterile saline, colistin methanesulfonate (CMS), CMS + Vitamin E + Vitamin C, and Vitamin E + vitamin C, respectively, for seven days. Thereafter, animals were sacrificed and the urine N-acetyl-b-D-glucosaminidase (NAG) and gamma-glutamyl transferase (GGT) levels, plasma level of creatinine (Cr), vitamin E and vitamin C, and renal tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as renal histology were performed. CMS induced acute tubular necrosis, increased the NAG, GGT, and MDA levels, and reduced the Vitamin E, Vitamin C, SOD, CAT, and GPx activities. Co-treatment with vitamins E and C restored all biochemical parameters cited above and improved the histopathological damage. Tubular damage induced by colistin is at least partly due to oxidative stress. Nephroprotective effect of Vitamins E and C is partially mediated through its antioxidant properties, and the higher protection by the combination of these vitamins is related to its synergistic effects.
本研究旨在探讨维生素E和维生素C联合使用对大鼠黏菌素诱导的肾小管损伤的保护作用。动物分别用无菌生理盐水、甲磺酸黏菌素（CMS）、CMS + 维生素E + 维生素C以及维生素E + 维生素C处理7天。此后，处死动物并检测尿N - 乙酰 - β - D - 氨基葡萄糖苷酶（NAG）和γ - 谷氨酰转移酶（GGT）水平、血浆肌酐（Cr）、维生素E和维生素C水平、肾组织丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）水平，以及进行肾脏组织学检查。CMS诱导急性肾小管坏死，增加NAG、GGT和MDA水平，并降低维生素E、维生素C、SOD、CAT和GPx活性。维生素E和维生素C联合处理可恢复上述所有生化参数，并改善组织病理学损伤。黏菌素诱导的肾小管损伤至少部分归因于氧化应激。维生素E和维生素C的肾保护作用部分通过其抗氧化特性介导，而这些维生素联合使用具有更高的保护作用与其协同效应有关。
