Specific Binding Protein ABCC1 Is Associated With Cry2Ab Toxicity in .

A pyramid strategy combining the crystal (Cry) 1A and 2A toxins in (Bt) crops are active against many species of insects and nematode larvae. It has been widely used to delay pest adaption to genetically modified plants and broaden the insecticidal spectrum in many countries. Unfortunately, Cry2A can also bind with the specific receptor proteins of Cry1A. ATP-binding cassette (ABC) transporters can interact with Cry1A toxins as receptors in the insect midgut, and ABC transporter mutations result in resistance to Bt proteins. However, there is limited knowledge of the ABC transporters that specifically bind to Cry2Ab. Here, we cloned the gene in , which expressed at all larval stages and in nine different tissues. Expression levels were particularly high in fifth-instar larvae and Malpighian tubules. The two heterologously expressed transmembrane domain peptides could specifically bind to Cry2Ab with high affinity levels. Moreover, transfecting into the nine insect cell significantly increased its mortality when exposed to Cry2Ab , and silencing in by RNA interference significantly reduced the mortality of larvae exposed to Cry2Ab . Altogether current results suggest that serves as a functional receptor for Cry2Ab.