NADH介导的基因表达以及Rex作为Rex调节子转录阻遏物的作用。
NADH-Mediated Gene Expression in and Role of Rex as a Transcriptional Repressor of the Rex-Regulon.
作者信息
Afzal Muhammad, Shafeeq Sulman, Kuipers Oscar P
机构信息
Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, Netherlands.
Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan.
出版信息
Front Microbiol. 2018 Jun 19;9:1300. doi: 10.3389/fmicb.2018.01300. eCollection 2018.
Nicotinamide adenine dinucleotides (NAD(H)) play a vital role in various biological processes, including keeping the cellular redox balance. In this study, we investigate the regulatory responses of D39 to NADH and characterize the role of the Rex protein as a transcriptional repressor of the , and genes. Transcriptomic analysis was used to observe the response of D39 to NADH. Our microarray studies revealed elevated expression of various genes/operons involved in transport and biosynthesis of niacin (, and ). Promoter -fusion assays and microarray studies with the mutant revealed the role of Rex as a transcriptional repressor of , and involved in niacin uptake and biosynthesis, in the presence of NADH. We predict the operator site (5'-TTGTKAWAAWWTTCACAA-3') of Rex in the regulatory regions of Rex-regulated genes that was subsequently validated by promoter mutational experiments.
烟酰胺腺嘌呤二核苷酸（NAD(H)）在各种生物过程中发挥着至关重要的作用，包括维持细胞氧化还原平衡。在本研究中，我们研究了D39对NADH的调节反应，并表征了Rex蛋白作为参与烟酸转运和生物合成的pncA、pncB和nadR基因转录阻遏物的作用。转录组分析用于观察D39对NADH的反应。我们的微阵列研究揭示了参与烟酸转运和生物合成的各种基因/操纵子（pncA、pncB和nadR）的表达升高。启动子融合分析以及对rex突变体的微阵列研究揭示了在NADH存在的情况下，Rex作为参与烟酸摄取和生物合成的pncA、pncB和nadR的转录阻遏物的作用。我们预测了Rex调控基因调控区域中Rex的操纵位点（5'-TTGTKAWAAWWTTCACAA-3'），随后通过启动子突变实验进行了验证。
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