Arora Y K, Sinha R C
J Bacteriol. 1985 Nov;164(2):811-5. doi: 10.1128/jb.164.2.811-815.1985.
Mycoplasmalike organisms (MLOs), purified from aster yellows-infected plants were osmotically lysed, and the membranes were separated from the cytoplasmic fraction through differential centrifugation. Electron microscopic examinations of sections of the purified MLOs and the isolated membranes showed pleomorphic bodies and unit membranous empty vesicles, respectively. Cell fractions were tested for NADH oxidase, NADPH oxidase, ATPase, RNase, DNase, and p-nitrophenyl phosphatase activity. NADH oxidase and ATPase were confined to the membrane fraction and NADPH oxidase to the cytoplasmic fraction of the MLOs. para-Nitrophenyl phosphatase, RNase, and DNase activities were detected in both membrane and cytoplasmic fractions, but p-nitrophenyl phosphatase and RNase appeared to be associated with membranes and DNase with the cytoplasmic fraction. Glucose-6-phosphate dehydrogenase was found in the cytoplasmic fraction of the MLO cells. Our findings on the distribution of enzymes in MLO cells and cell fractions are the first basic documentation on nonhelical, nonculturable microbes parasitic to plants.
从感染翠菊黄化病的植物中纯化出类支原体生物（MLOs），将其进行渗透裂解，然后通过差速离心将膜与细胞质组分分离。对纯化后的MLOs切片和分离出的膜进行电子显微镜检查，分别显示出多形性菌体和单位膜性空泡。对细胞组分进行了NADH氧化酶、NADPH氧化酶、ATP酶、核糖核酸酶、脱氧核糖核酸酶和对硝基苯磷酸酶活性检测。NADH氧化酶和ATP酶局限于MLOs的膜组分，而NADPH氧化酶局限于细胞质组分。在膜组分和细胞质组分中均检测到对硝基苯磷酸酶、核糖核酸酶和脱氧核糖核酸酶活性，但对硝基苯磷酸酶和核糖核酸酶似乎与膜相关，而脱氧核糖核酸酶与细胞质组分相关。在MLO细胞的细胞质组分中发现了葡萄糖-6-磷酸脱氢酶。我们关于MLO细胞和细胞组分中酶分布的研究结果是关于寄生在植物上的非螺旋状、不可培养微生物的第一份基础文献。
