解脲脲原体的细胞组分及酶活性
Cell fractions and enzymatic activities of Ureaplasma urealyticum.
作者信息
Romano N, La Licata R
出版信息
J Bacteriol. 1978 Dec;136(3):833-8. doi: 10.1128/jb.136.3.833-838.1978.
Abstract
The localization of some enzymic activities in cell fractions of Ureaplasma urealyticum was studied. A quantitative evaluation of the effectiveness of several cell lysis procedures was obtained by using labeled membranes and sucrose density gradient centrifugation. Ultrasonic treatment was found to be the most effective procedure for lysing the cells, whereas digitonin and osmotic shock caused the lysis of only 70 and 50% of the cells, respectively. The localization of selected enzymes in Ureaplasma cells resembled that found in other Mycoplasma species. Adenosine triphosphatase, ribonuclease, deoxyribonuclease, and p-nitrophenylphosphatase activities were located exclusively in the membrane fraction, whereas urease and L-histidine ammonia-lyase were located in the cytoplasm.
摘要
研究了解脲脲原体细胞组分中某些酶活性的定位。通过使用标记膜和蔗糖密度梯度离心法，对几种细胞裂解程序的有效性进行了定量评估。发现超声处理是裂解细胞最有效的程序，而洋地黄皂苷和渗透压休克分别仅导致70%和50%的细胞裂解。所选酶在脲原体细胞中的定位与在其他支原体物种中发现的相似。三磷酸腺苷酶、核糖核酸酶、脱氧核糖核酸酶和对硝基苯磷酸酶活性仅位于膜组分中，而脲酶和L-组氨酸解氨酶位于细胞质中。
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