利用大肠杆菌乳糖通透酶基因产物测量苜蓿中华根瘤菌的质子动力。
Measurement of the proton motive force in Rhizobium meliloti with the Escherichia coli lacY gene product.
作者信息
Gober J W, Kashket E R
出版信息
J Bacteriol. 1985 Nov;164(2):929-31. doi: 10.1128/jb.164.2.929-931.1985.
Abstract
An Escherichia coli lac operon constitutive for lacY was subcloned into the EcoRI site of a wide-host-range plasmid of the Q incompatibility group, and the resulting recombinant plasmid was introduced into Tn5-generated Lac- mutants of Rhizobium meliloti. The R. meliloti transconjugants accumulated lactose about 1,000-fold, equivalent to a proton motive force of -170 to -180 mV, not significantly different from the values calculated from the distributions of weak acids and lipophilic cations.
摘要
一个对lacY组成型的大肠杆菌乳糖操纵子被亚克隆到Q不相容群的一个广宿主范围质粒的EcoRI位点，然后将所得的重组质粒导入苜蓿根瘤菌的Tn5诱导产生的Lac-突变体中。苜蓿根瘤菌转接合子积累的乳糖量约为原来的1000倍，相当于-170至-180 mV的质子动力势，这与根据弱酸和亲脂性阳离子分布计算的值没有显著差异。
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