转座乳糖操纵子Tn951在球形红假单胞菌中的表达。
Expression of the transposable lac operon Tn951 in Rhodopseudomonas sphaeroides.
作者信息
Nano F E, Kaplan S
出版信息
J Bacteriol. 1982 Nov;152(2):924-7. doi: 10.1128/jb.152.2.924-927.1982.
Abstract
The transposon Tn951 (lac) was introduced into the photosynthetic bacterium Rhodopseudomonas sphaeroides 2.4.1, which is normally Lac-, via the P-group plasmid RP1. beta-Galactosidase was produced constitutively in both chemotrophically and phototrophically grown cells, and the levels were found to be the same but low. Mutants were isolated, however, that were able to grow on lactose minimal medium and which expressed different levels of beta-galactosidase when grown chemotrophically or phototrophically. The beta-galactosidase levels found in all R. sphaeroides strains were much less than those found in Escherichia coli.
摘要
转座子Tn951（lac）通过P群质粒RP1被导入光合细菌球形红假单胞菌2.4.1中，该细菌通常为乳糖阴性。在化学营养生长和光合营养生长的细胞中均组成型产生β-半乳糖苷酶，且发现其水平相同但较低。然而，分离出了一些突变体，它们能够在乳糖基本培养基上生长，并且在化学营养生长或光合营养生长时表达不同水平的β-半乳糖苷酶。在所有球形红假单胞菌菌株中发现的β-半乳糖苷酶水平远低于在大肠杆菌中发现的水平。
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