Li Kaige, Guo Mengwei, Tan Lihua, Li Xiaohong, Wu Yanying, Lan Ying, Zhu Wenlian, Zhao Yafang, Zhang Lufen, Ren Xiaoxuan
School of Acupuncture, Moxibustion and Tuina, Beijing University of CM, Beijing 100029, China; Beijing Kangyide Pulmonary Hospital of Combined Western Medicine and TCM, Beijing 101400.
School of Acupuncture, Moxibustion and Tuina, Beijing University of CM, Beijing 100029, China.
Zhongguo Zhen Jiu. 2018 Jun 12;38(6):625-9. doi: 10.13703/j.0255-2930.2018.06.016.
OBJECTIVE: To compare the effects of electroacupuncture (EA) at "Dachangshu" (BL 25) or "Tianshu" (ST 25) for visceral sensitivity, gene expression product c-kit of colonic Cajal interstitial cells (ICC) and capsaicin receptor 1 (TRPV1) of irritable bowel syndrome (IBS) rats, so as to investigate the effect and mechanism differences of EA at the back point and the front point of large intestine for IBS rats. METHODS: Forty-two Wistar neonatal rats were randomly divided into a blank group (9 rats) and a model group (33 rats). IBS model was established with mother and child separation, acetic acid enema in young rats and colorectal dilatation method. Twenty-seven IBS rats in life were randomly divided into a model control group, a Dachangshu group and a Tianshu group, 9 rats in each group. EA (disperse-dense wave, 2 Hz/100 Hz, 0.1-0.3 mA) for 20 min was used at "Dachangshu" (BL 25) and "Tianshu" (ST 25) respectively in the Dachangshu and Tianshu groups, once every other day, totally 5 times. The rats in the model control group were fixed with soft cloth sleeve for 20 min, without acupuncture. No intervention was used in the blank group. The stool property Bristol grading score was recorded before and after intervention in each group. The visceral sensitivity was evaluated by abdominal withdrawal reflex. The latency until the first systolic wave occurred and the number of systolic wave within 90 s were observed. Immunohistochemical was used to detect the positive expressions of c-kit and TRPV1, the ICC colon specific marker. RESULTS: Compared with the blank group, the Bristol score increased，latency period shortened, systolic wave number increased, c-kit and TRPV1 positive expressions increased in the model control group (all <0.01). Compared with the model control group, the Bristol score decreased, latency period increased, systolic wave number decreased, c-kit and TRPV1 positive expressions decreased after intervention in the Dachangshu and Tianshu groups (<0.05, <0.01). Compared with the Dachangshu group, the TRPV1 positive expression decreased after intervention in the Tianshu group (<0.05). CONCLUSION: EA at "Dachangshu" (BL 25) or "Tianshu"(ST 25) can improve the diarrhea in IBS model rats, reduce the visceral sensitivity, and its mechanism may be related to regulating the expressions of colon c-kit and TRPV1. EA at "Tianshu" (ST 25) is more apparent for TRPV1 than at "Dachangshu" (BL 25).
目的：比较电针“大肠俞”（BL 25）或“天枢”（ST 25）对肠易激综合征（IBS）大鼠内脏敏感性、结肠Cajal间质细胞（ICC）基因表达产物c-kit和辣椒素受体1（TRPV1）的影响，以探讨大肠经背部穴位与腹部穴位电针对IBS大鼠作用及机制的差异。 方法：42只Wistar新生大鼠随机分为空白组（9只）和模型组（33只）。采用母子分离、幼鼠乙酸灌肠及结直肠扩张法建立IBS模型。将造模成功的27只IBS大鼠随机分为模型对照组、大肠俞组和天枢组，每组9只。大肠俞组和天枢组分别于“大肠俞”（BL 25）和“天枢”（ST 25）采用电针（疏密波，2 Hz/100 Hz，0.1 - 0.3 mA）20 min，隔日1次，共5次。模型对照组用软布套固定20 min，不行针刺。空白组不做干预。记录各组干预前后粪便性状布里斯托分级评分。通过腹部退缩反射评估内脏敏感性。观察首次出现收缩波的潜伏期及90 s内收缩波的次数。采用免疫组织化学法检测结肠特异性标志物ICC的c-kit和TRPV1的阳性表达。 结果：与空白组比较，模型对照组布里斯托评分升高，潜伏期缩短，收缩波次数增加，c-kit和TRPV1阳性表达增加（均P<0.01）。与模型对照组比较，大肠俞组和天枢组干预后布里斯托评分降低，潜伏期延长，收缩波次数减少，c-kit和TRPV1阳性表达降低（P<0.05，P<0.01）。与大肠俞组比较，天枢组干预后TRPV1阳性表达降低（P<0.05）。 结论：电针“大肠俞”（BL 25）或“天枢”（ST 25）可改善IBS模型大鼠腹泻，降低内脏敏感性，其机制可能与调节结肠c-kit和TRPV1的表达有关。电针“天枢”（ST 25）对TRPV1的影响较“大肠俞”（BL 25）更明显。
Zhongguo Zhen Jiu. 2022-12-12
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