Yang Yi-Chen, Zhou Zi-Xian, Xue Ting, Feng Yuan-Hui, Chen Jun-Tao, Wang Tu-Nan, Zhao Jia-Yu, Wang Liu-Jing, Zhang Peng, Zhang Li-Ping, Ma Hui-Fang
School of Acupuncture-Moxibustion and Tuina, Beijing University of CM, Beijing 100029, China.
School of TCM Health Service, Shanxi Datong University.
Zhongguo Zhen Jiu. 2022 Dec 12;42(12):1395-402. doi: 10.13703/j.0255-2930.20220130-0005.
To observe the effect of electroacupuncture (EA) at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) on mental state, visceral sensitivity and protein expression of nerve growth factor (NGF), tyrosine kinase receptor A (TrkA) and transient receptor potential vanilloid 1 (TRPV1) of colonic tissue in diarrhea-predominant irritable bowel syndrome (IBS-D) rats, and to explore its possible mechanism on treating IBS-D.
A total of 36 male SD rats of SPF grade were randomized into a blank group, a model group, an EA group and a western medication group, 9 rats in each group. In the model group, the EA group and the western medication group, IBS-D model was established by enema of dinitrobenzene sulfonic acid (DNBS) combined with chronic restraint stress method. In the EA group, EA was applied at "Tianshu" (ST 25) and "Shangjuxu" (ST 37), with disperse-dense wave, in frequency of 2 Hz/100 Hz, 20 min each time, once a day for 7 days. In the western medication group, pinaverium bromide suspension was given by gavage (15 mg•kg•d) for 7 days. Before and after model establishment, and after intervention, the body mass, 24 h food intake and fecal water content were observed, the visceral sensitivity was detected by abdominal withdrawal reflex (AWR); after intervention, the mental state was evaluated by elevated plus maze (EPM) test, the protein expression of NGF, TrkA and TRPV1 was detected by immunohistochemistry and Western blot in the 4 groups.
After model establishment, compared with the blank group, the body mass and 24 h food intake were decreased (<0.05), first systolic latency of AWR was shortened and number of contraction wave of AWR was increased (<0.05), and fecal water content was increased (<0.05) in the model group, the EA group and the western medication group. After intervention, compared with the blank group, open arm residence time ratio (OT%) of EPM was decreased (<0.05) and protein expression of NGF, TrkA, TRPV1 in colonic tissue was increased in the model group (<0.05); compared with the model group, the body mass and 24 h food intake were increased (<0.05), first systolic latency of AWR was lengthened and number of contraction wave of AWR was decreased (<0.05), the fecal water content was decreased (<0.05), OT% of EPM was increased (<0.05), and protein expression of NGF, TrkA, TRPV1 in colonic tissue was decreased (<0.05) in the EA group and the western medication group.
Electroacupuncture at "Tianshu" (ST 25) and "Shangjuxu" (ST 37) can relieve the anxiety and depression-like behaviors in IBS-D rats, down-regulate the protein expression of NGF, TrkA, TRPV1 in colonic tissue, so as to reduce the visceral sensitivity and relieve symptoms.
观察电针“天枢”（ST25）、“上巨虚”（ST37）对腹泻型肠易激综合征（IBS-D）大鼠精神状态、内脏敏感性及结肠组织神经生长因子（NGF）、酪氨酸激酶受体A（TrkA）和瞬时受体电位香草酸受体1（TRPV1）蛋白表达的影响，探讨其治疗IBS-D的可能机制。
将36只SPF级雄性SD大鼠随机分为空白组、模型组、电针组和西药组，每组9只。模型组、电针组和西药组采用二硝基苯磺酸（DNBS）灌肠结合慢性束缚应激法建立IBS-D模型。电针组针刺“天枢”（ST25）、“上巨虚”（ST37），采用疏密波，频率为2Hz/100Hz，每次20min，每日1次，连续7d。西药组给予匹维溴铵混悬液灌胃（15mg•kg•d），连续7d。观察造模前后及干预后大鼠体质量、24h摄食量及粪便含水量，采用腹部撤免反射（AWR）检测内脏敏感性；干预后采用高架十字迷宫（EPM）试验评价精神状态，免疫组化和Western blot法检测4组大鼠结肠组织中NGF、TrkA和TRPV1蛋白表达。
造模后，与空白组比较，模型组、电针组和西药组大鼠体质量和24h摄食量降低（P<0.05），AWR首次收缩潜伏期缩短、收缩波次数增加（P<0.05），粪便含水量增加（P<0.05）。干预后，与空白组比较，模型组EPM开臂停留时间比率（OT%）降低（P<0.05），结肠组织中NGF、TrkA、TRPV1蛋白表达增加（P<0.05）；与模型组比较，电针组和西药组大鼠体质量和24h摄食量增加（P<0.05），AWR首次收缩潜伏期延长、收缩波次数减少（P<0.05），粪便含水量降低（P<0.05），EPM的OT%升高（P<0.05），结肠组织中NGF、TrkA、TRPV1蛋白表达降低（P<0.05）。
电针“天枢”（ST25）、“上巨虚”（ST37）可缓解IBS-D大鼠的焦虑抑郁样行为，下调结肠组织中NGF、TrkA、TRPV1蛋白表达，从而降低内脏敏感性，缓解症状。
