Ji Mao-Xian, Guo Meng-Wei, Gao Yu-Shan, Lan Ying, Wang Shan, Wang Yi-Fan, Qin Ying, Zhang Hong-Lin, Ren Xiao-Xuan
School of Acupuncture-moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China.
Zhen Ci Yan Jiu. 2019 Apr 25;44(4):264-9. doi: 10.13702/j.1000-0607.180764.
To observe and compare the effect of electroacupuncture (EA) of "Tianshu" (ST25) and "Dachangshu" (BL25) on intestinal sensitivity and the expression of M muscarinic acetylcholine receptor (MR) and 5-hydroxytryptamine 3A receptor (5-HTR) in the colon tissue in irritable bowel syndrome (IBS) rats, so as to explore its mechanism underlying improvement of IBS.
Forty newborn Wistar rats were randomly divided into blank control, model, ST25 and BL25 groups (＝10 rats per group). The IBS model was established by joint application of maternal separation, acetic acid enema plus colorectal distension from day 8 to 21 after birth. At the age of 9 weeks, EA (2 Hz/100 Hz, 0.1－0.3 mA) was applied to ST25 and BL25 for 20 min, once every other day for 5 times. The modified method was used to record the abdominal withdrawal reflex (AWR), so as to evaluate the visceral sensitivity by referring to the modified Al-Chaer's and colleagues' methods. The threshold (initial wave), the number and peak-peak value of contraction waves (AWR) within 90 s were recorded. The immunoactivity of MR and 5-HTR in the colon tissue was detected by immunohistochemistry.
After modeling, the latency was obviously shortened, the number and peak-peak values of contraction waves of intestine were significantly increased, and the immunoactivity of MR and 5-HTR was notably up-regulated in the model group compared with the blank control group (<0.01). After the EA intervention, the latency was obviously prolonged, the peak-peak value in both ST25 and BL25 groups, and the number of contraction waves in the ST25 group were significantly decreased (<0.01), and the expression levels of M and 5-HT R were considerably down-regulated in both ST25 and BL25 groups relevant to the model group (<0.01). The therapeutic effects were significantly better in the ST25 group than in the BL25 group in prolonging the latency and reducing the contraction wave number, and in down-regulating the expression of colonic MR and 5-HTR (<0.05, <0.01).
EA at ST25 and BL25 can reduce the intestinal sensitivity in IBS rats, which is probably associated with its effects in down-regulating the expression of MR and 5-HTR in the colon. The therapeutic effects of ST25 were obviously better than those of BL25 in suppressing intestinal sensitivity and expression of colonic MR and 5-HTR.
观察并比较电针“天枢”（ST25）和“大肠俞”（BL25）对肠易激综合征（IBS）大鼠肠道敏感性及结肠组织中M型毒蕈碱型乙酰胆碱受体（MR）和5-羟色胺3A受体（5-HTR）表达的影响，以探讨其改善IBS的作用机制。
40只新生Wistar大鼠随机分为空白对照组、模型组、ST25组和BL25组（每组10只）。于出生后第8至21天采用母婴分离、乙酸灌肠加结肠扩张联合应用的方法建立IBS模型。9周龄时，对ST25和BL25进行电针治疗（2 Hz/100 Hz，0.1－0.3 mA），每次20分钟，隔日1次，共5次。采用改良方法记录腹部回撤反射（AWR），参照改良的Al-Chaer等人的方法评估内脏敏感性。记录阈值（起始波）、90秒内收缩波的数量和峰峰值（AWR）。采用免疫组织化学法检测结肠组织中MR和5-HTR的免疫活性。
造模后，与空白对照组比较，模型组潜伏期明显缩短，肠收缩波数量和峰峰值显著增加，MR和5-HTR的免疫活性显著上调（<0.01）。电针干预后，潜伏期明显延长，ST25组和BL25组的峰峰值以及ST25组的收缩波数量均显著减少（<0.01），与模型组比较，ST25组和BL25组M和5-HT R的表达水平均显著下调（<0.01）。在延长潜伏期、减少收缩波数量以及下调结肠MR和5-HTR表达方面，ST25组的治疗效果显著优于BL25组（<0.05，<0.01）。
电针ST25和BL25可降低IBS大鼠的肠道敏感性，这可能与其下调结肠中MR和5-HTR的表达有关。在抑制肠道敏感性以及结肠MR和5-HTR表达方面，ST25的治疗效果明显优于BL25。
