从感染的LSTRA淋巴瘤细胞产生的水疱性口炎病毒具有酪氨酸蛋白激酶活性（p56）。
Vesicular stomatitis virus produced from infected LSTRA lymphoma cells bear tyrosine protein kinase activity (p56).
作者信息
Fischer S, Flamand A, Fagard R, Chich J F, Piau J P, Reibel L, Gacon G
出版信息
J Biol Chem. 1985 Nov 25;260(27):14406-9.
Murine LSTRA lymphoma cells contain a very active tyrosine protein kinase of 56 kDa (p56) which is not related to any of the other known tyrosine kinases. In the past the purification and characterization of the p56 have been hampered because of the low amount of this protein in LSTRA membranes. In this study, we have utilized a different approach for purification which consisted of trapping the protein in the membrane of vesicular stomatitis virus. Incubation of the virions with [gamma-32P]ATP resulted in the phosphorylation of p56 on tyrosine residues. Moreover, the phosphopeptide digest profile of vesicular stomatitis virus-p56 was identical to that observed with authentic LSTRA-p56. The p56 from such virions could be resolved from other proteins by two-dimensional gels, and furthermore, such virions have been used to prepare several antisera directed against the p56.
小鼠LSTRA淋巴瘤细胞含有一种非常活跃的56 kDa酪氨酸蛋白激酶（p56），它与任何其他已知的酪氨酸激酶均无关联。过去，由于LSTRA细胞膜中这种蛋白质的含量较低，p56的纯化和特性鉴定受到了阻碍。在本研究中，我们采用了一种不同的纯化方法，即将该蛋白质截留在水疱性口炎病毒的膜中。用[γ-32P]ATP孵育病毒粒子会导致p56的酪氨酸残基发生磷酸化。此外，水疱性口炎病毒-p56的磷酸肽消化图谱与用天然LSTRA-p56观察到的图谱相同。通过二维凝胶电泳可以将此类病毒粒子中的p56与其他蛋白质区分开来，此外，此类病毒粒子已被用于制备几种针对p56的抗血清。
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