Clinton G M, Guerina N G, Guo H Y, Huang A S
J Biol Chem. 1982 Mar 25;257(6):3313-9.
Among the protein kinases associated with vesicular stomatitis virus (VSV), one was identified by immunoprecipitation to be pp60src, the transformation-specific product coded for by avian sarcoma virus, or its endogenous cellular homolog. This activity phosphorylated only tyrosine. pp60src was enriched in the membranes, whereas the serine- and threonine-specific kinases were concentrated with viral cores. The content of pp60src in VSV can be manipulated by growing VSV in different host cells. Monolayer baby hamster kidney cells transformed by an avian sarcoma virus produced VSV progeny which contained 7-fold greater pp60src activity than progeny produced by control untransformed or revertant cells. In contrast, suspension cultures of baby hamster kidney cells which produced VSV with increased tyrosine-specific kinase activity did not affect the content of pp60src. When pp60src was specifically increased in cells, the endogenous phosphorylation of tyrosine residues in the VSV matrix M protein was also enhanced, to as much as 20-fold. The phosphorylation of serine or threonine in this protein or in the other VSV phosphoprotein NS was not affected. Cellular tyrosine-specific kinases other than pp60scr did not change the overall phosphorylation pattern of any VSV phosphoproteins. Experiments designed to test the effects of endogenous phosphorylation on the various functions of the M protein failed to detect any significant alterations.
在与水泡性口炎病毒（VSV）相关的蛋白激酶中，有一种通过免疫沉淀法被鉴定为pp60src，它是禽肉瘤病毒编码的转化特异性产物，或是其细胞内同源物。这种活性仅使酪氨酸磷酸化。pp60src在膜中富集，而丝氨酸和苏氨酸特异性激酶则集中在病毒核心部位。VSV中pp60src的含量可通过在不同宿主细胞中培养VSV来调控。经禽肉瘤病毒转化的单层幼仓鼠肾细胞产生的VSV子代，其pp60src活性比未转化的对照细胞或回复突变细胞产生的子代高7倍。相比之下，产生具有增强酪氨酸特异性激酶活性的VSV的幼仓鼠肾细胞悬浮培养物，并不影响pp60src的含量。当细胞中pp60src特异性增加时，VSV基质M蛋白中酪氨酸残基的内源性磷酸化也增强，最高可达20倍。该蛋白或其他VSV磷蛋白NS中丝氨酸或苏氨酸的磷酸化不受影响。除pp60scr外的细胞酪氨酸特异性激酶不会改变任何VSV磷蛋白的整体磷酸化模式。旨在测试内源性磷酸化对M蛋白各种功能影响的实验未能检测到任何显著变化。
