Kamel Azza M, El-Fishawi Sally, Rasekh Eman O, Radwan Eman R, Zeidan Ashraf, El-Said Abeer, Zaky Amen H, Abdelfattah Maged, Refaat Ahmed, Abdelfattah Raafat
a Clinical Pathology Department , NCI, Cairo University , Cairo , Egypt.
b Faculty of Medicine, Clinical and Chemical Pathology Department , Cairo University , Cairo , Egypt.
Leuk Lymphoma. 2018 Dec;59(12):2963-2972. doi: 10.1080/10428194.2018.1459608. Epub 2018 Jul 4.
Graft-versus-host disease (GVHD) remains one of the major complications of hematopoietic stem cell transplantation (HSCT). Several etiological factors were investigated. Among these, vitamin D and hence its receptor (VDR) gene polymorphisms have gained much interest; however, the results are still controversial. Using PCR-RFLP, we genotyped VDR polymorphisms FokI (rs10735810), ApaI (rs7975232), and Taq1 (rs731236) in 80 patient/donor pairs according to DNA availability. No association was encountered between VDR polymorphisms and GVHD. Neither was there any impact on survival. Only grade II-IV acute GVHD was associated with inferior overall (p = .01), but not disease-free survival. The controversy between our results and the literature may be attributed to marked variability in the relative distribution of VDR genotypes in different populations. Also different environmental factors, including exposure to sun, may ensure vitamin D sufficiency nullifying the impact of VDR polymorphisms.
移植物抗宿主病（GVHD）仍然是造血干细胞移植（HSCT）的主要并发症之一。研究了几种病因。其中，维生素D及其受体（VDR）基因多态性备受关注；然而，结果仍存在争议。我们根据DNA可得性，运用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术，对80对患者/供体的VDR多态性FokI（rs10735810）、ApaI（rs7975232）和Taq1（rs731236）进行基因分型。未发现VDR多态性与GVHD之间存在关联。对生存率也没有任何影响。只有II-IV级急性GVHD与总体生存率较低相关（p = 0.01），但与无病生存率无关。我们的结果与文献之间的争议可能归因于不同人群中VDR基因型相对分布的显著差异。此外，包括阳光照射在内的不同环境因素可能确保维生素D充足，从而消除VDR多态性的影响。
