Performance of the IS6110-TaqMan® assay in the diagnosis of extrapulmonary tuberculosis from different biological samples.
作者信息
Santos Fabiana Cristina Fulco, Lira Laís Ariane de Siqueira, Montenegro Rosana de Albuquerque, Lima Juliana Figueirêdo da Costa, Lima Andrea Santos, Schindler Haiana Charifker, Montenegro Lílian Maria Lapa
机构信息
Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brasil.
出版信息
Rev Soc Bras Med Trop. 2018 May-Jun;51(3):331-337. doi: 10.1590/0037-8682-0372-2017.
INTRODUCTION
This study evaluated the performance of the IS6110-TaqMan® assay in different types of biological samples and tissues for laboratory diagnosis of extrapulmonary tuberculosis.
METHODS
143 biological samples and tissues from patients with suspected extrapulmonary tuberculosis from the health services of Recife/Pernambuco/Brazil were evaluated with the IS6110-TaqMan® assay.
RESULTS
The sensitivities of the IS6110-TaqMan® assay calculated for blood, urine, both blood and urine samples, tissue biopsies, extrapulmonary body fluid samples, and all samples from patients calculated together were 55.9%, 33.3%, 68.8%, 43.8%, 29.6%, and 73.7%, respectively, and the specificities were 80%, 100%, 78.6%, 100%, 100%, and 84.2%, respectively.
CONCLUSIONS
The accuracy of qPCR was high in various clinical sample types. The analysis of more than one type of clinical sample collected from the same patient with extrapulmonary tuberculosis enhances the diagnostic power of the IS6110-TaqMan® assay when compared with the use of only one clinical sample.
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