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犬钩端螺旋体属的血清学和分子检测

Serological and molecular detection of Leptospira spp in dogs.

作者信息

Latosinski Giulia Soares, Fornazari Felipe, Babboni Selene Daniela, Caffaro Karen, Paes Antonio Carlos, Langoni Helio

机构信息

Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho, Botucatu, SP, Brasil.

出版信息

Rev Soc Bras Med Trop. 2018 May-Jun;51(3):364-367. doi: 10.1590/0037-8682-0276-2017.

DOI:10.1590/0037-8682-0276-2017
PMID:29972570
Abstract

INTRODUCTION

This study aimed to detect anti-Leptospira spp antibodies and Leptospira DNA in domestic dogs.

METHODS

Blood and urine from 106 dogs were evaluated by microscopic agglutination test (MAT) and polymerase chain reaction (PCR), respectively.

RESULTS

Six (5.7%) and one (1%) animals were positive by MAT and PCR, respectively.

CONCLUSIONS

These results show a low prevalence of infection by Leptospira spp. The absence of positive results for the Icterohaemorrhagiae serogroup indicates the small relevance of these dogs as sources of human leptospirosis.

摘要

引言

本研究旨在检测家犬体内的抗钩端螺旋体属抗体和钩端螺旋体DNA。

方法

分别采用显微镜凝集试验(MAT)和聚合酶链反应(PCR)对106只犬的血液和尿液进行评估。

结果

MAT和PCR检测分别有6只(5.7%)和1只(1%)动物呈阳性。

结论

这些结果表明钩端螺旋体属感染的患病率较低。出血性黄疸型血清群未出现阳性结果,表明这些犬作为人类钩端螺旋体病传染源的相关性较小。

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