印度哈里亚纳邦通过定量聚合酶链反应检测看似健康的犬类尿液中的[具体物质未给出] 。
Detection of in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India.
作者信息
Rohilla Preeti, Khurana Rajesh, Kumar Aman, Batra Kanisht, Gupta Renu
机构信息
Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
出版信息
Vet World. 2020 Nov;13(11):2411-2415. doi: 10.14202/vetworld.2020.2411-2415. Epub 2020 Nov 12.
BACKGROUND AND AIM
Leptospirosis is a zoonotic disease caused by bacteria of the genus . The organism can spread through the urine of infected animals, which can get into water or soil and can survive there for weeks to months. The study was undertaken to detect the pathogenic in healthy dogs' urinary shedding by real-time polymerase chain reaction (qPCR).
MATERIALS AND METHODS
Leptospirosis is a zoonotic disease caused by bacteria of the genus . To detect the pathogenic organisms in dogs' urinary shedding, 239 urine samples were collected from healthy dogs from April 2018 to March 2019 from different areas of Haryana. All the urine samples were processed for DNA extraction and qPCR technique was used to detect the presence of .
RESULTS
Out of 239 urine samples of dogs, none of the samples resulted in the detection of DNA of pathogenic organisms.
CONCLUSION
The present study indicated low risk of transmission of organisms from dogs' urine to human beings in Haryana.
背景与目的
钩端螺旋体病是一种由钩端螺旋体属细菌引起的人畜共患病。该病原体可通过感染动物的尿液传播，尿液进入水或土壤后可在其中存活数周至数月。本研究旨在通过实时聚合酶链反应（qPCR）检测健康犬尿液中排出的致病钩端螺旋体。
材料与方法
钩端螺旋体病是一种由钩端螺旋体属细菌引起的人畜共患病。为检测犬尿液中排出的致病钩端螺旋体，于2018年4月至2019年3月从哈里亚纳邦不同地区的健康犬采集了239份尿液样本。所有尿液样本均进行DNA提取处理，并采用qPCR技术检测钩端螺旋体的存在。
结果
在239份犬尿液样本中，未检测到致病钩端螺旋体的DNA。
结论
本研究表明，在哈里亚纳邦，犬尿液中的钩端螺旋体传播给人类的风险较低。
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