Wu W C, Chen S S, Wang K, Yang Y, Wu Y F, Zhao L
Department of Periodontology, West China Hospital of Stomatology, Sichuan University & State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases(Present address: Department of Periodontology, The Affiliated Stomatological Hospital of Nanchang University & The Key Laboratory of Oral Biomedicine, Jiangxi Province, Nanchang 330006, China).
Department of Periodontology, West China Hospital of Stomatology, Sichuan University & State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases (Present address: Medical Center of Stomatology, Beijing An Zhen Hospital, Capital Medical University, Beijing 100029, China).
Zhonghua Kou Qiang Yi Xue Za Zhi. 2018 Mar 9;53(3):157-163. doi: 10.3760/cma.j.issn.1002-0098.2018.03.003.
To illuminate the temporal expression of the triggering receptor expressed on myeloid cells-1 (TREM-1) in the experimental periodontitis in rat and to investigate the function of TREM-1 in the pathogenesis of experimental periodontitis in rat. The experimental periodontitis model was established in the maxillary first molar by means of 'wire ligation + vaccination periodontal pathogen (Pg) + high-sugar diet' in Sprague-Dawley (SD) rats. The experimental animals were divided into six groups: the control group and each of the time points of establishing the models for one week and two to five weeks. There were six rats for each of the six groups. The bone loss of the palatal site was calculated to estimate whether the periodontitis model was successfully established. The expression of TREM-1, proinflammatory cytokines: tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6, anti-inflammatory cytokines: IL-4, IL-10 and transforming growth factor-β (TGF-β) were examined by using quantitative real-time PCR. The expression level of TREM-1 protein was analyzed by the method of immunohistochemistry. The average bone loss area of the palatal site was (0.17±0.04) mm(2) in the group of three weeks and was statistically significant (0.05) compared to the control group [(0.10±0.01) mm(2)]. The experimental periodontitis model was successfully established in the group of three weeks. The expression of TREM-1 increased significantly in the inflamed periodontal tissues and reached to its maximum expression in the three weeks group accounting for 159.50±38.26 in protein expression and 4.35±0.60 in mRNA expression, respectively. TREM-1 expression difference between the three weeks group and control group was statistically significant (0.01). The expression of IL-6 by gingival tissues was correlated with the mRNA level of TREM-1 (0.813 0.049). TREM-1, as a proinflammatory receptor, could facilitate the periodontal inflammatory response. The possible way of TREM-1 to promote inflammation may be through controling the expression of IL-6.
为阐明髓样细胞触发受体-1（TREM-1）在大鼠实验性牙周炎中的时序表达，并探讨TREM-1在大鼠实验性牙周炎发病机制中的作用。采用“丝线结扎+接种牙周病原菌（Pg）+高糖饮食”的方法在Sprague-Dawley（SD）大鼠上颌第一磨牙建立实验性牙周炎模型。将实验动物分为六组：对照组以及模型建立后1周、2至5周各时间点组。六组每组各6只大鼠。计算腭侧部位的骨吸收量以评估牙周炎模型是否成功建立。采用定量实时PCR检测TREM-1、促炎细胞因子：肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-1β和IL-6、抗炎细胞因子：IL-4、IL-10和转化生长因子-β（TGF-β）的表达。采用免疫组织化学方法分析TREM-1蛋白的表达水平。三周组腭侧部位的平均骨吸收面积为（0.17±0.04）mm²，与对照组[（0.10±0.01）mm²]相比具有统计学意义（P<0.05）。三周组成功建立了实验性牙周炎模型。TREM-1在炎症牙周组织中的表达显著增加，在三周组达到最大表达，蛋白表达为159.50±38.26，mRNA表达为4.35±0.60。三周组与对照组之间TREM-1表达差异具有统计学意义（P<0.01）。牙龈组织中IL-6的表达与TREM-1的mRNA水平相关（r=0.813，P=0.049）。TREM-1作为一种促炎受体，可促进牙周炎症反应。TREM-1促进炎症的可能途径可能是通过调控IL-6的表达。
