Danihlík Jiří, Škrabišová Mária, Lenobel René, Šebela Marek, Omar Eslam, Petřivalský Marek, Crailsheim Karl, Brodschneider Robert
Department of Protein Biochemistry and Proteomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Department of Biochemistry, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, 783 71 Olomouc, Czech Republic.
Insects. 2018 Jul 4;9(3):79. doi: 10.3390/insects9030079.
We investigated the importance of protein nutrition for honey bee immunity. Different protein diets (monofloral pollen of spp., spp., spp., spp., a mixture of the four different pollen and the pollen substitute Feedbee) were fed to honey bees in cages . After 18 days of feeding, apidaecin 1 isoforms concentration in the thorax were measured using nanoflow liquid chromatography coupled with mass spectrometry. Expression levels of genes, coding for apidaecins and abaecin in the abdomen were determined using quantitative PCR. The results indicate that protein-containing nutrition in adult worker honey bees can trigger certain metabolic responses. Bees without dietary protein showed lower apidaecin 1 isoforms concentrations. The significantly lowest concentration of apidaecin 1 isoforms was found in the group that was fed no pollen diet when compared to , , , and pollen or the pollen supplement FeedBee. Expression levels of the respective genes were also affected by the protein diets and different expression levels of these two antimicrobial peptides were found. Positive correlation between concentration and gene expression of apidaecins was found. The significance of feeding bees with different protein diets, as well as the importance of pollen nutrition for honey bee immunity is demonstrated.
我们研究了蛋白质营养对蜜蜂免疫力的重要性。将不同的蛋白质饲料（来自[具体植物种类1]、[具体植物种类2]、[具体植物种类3]、[具体植物种类4]的单花花粉、四种不同花粉的混合物以及花粉替代物Feedbee）喂给笼中的蜜蜂。喂食18天后，使用纳流液相色谱-质谱联用技术测量蜜蜂胸部中蜂防御素1同工型的浓度。使用定量PCR测定腹部中编码蜂防御素和阿贝辛的基因的表达水平。结果表明，成年工蜂的含蛋白质营养可引发某些代谢反应。无蛋白质饮食的蜜蜂显示出较低的蜂防御素1同工型浓度。与[具体植物种类1]、[具体植物种类2]、[具体植物种类3]、[具体植物种类4]花粉或花粉补充物FeedBee相比，未喂食花粉饮食的组中蜂防御素1同工型的浓度显著最低。相应基因的表达水平也受蛋白质饲料的影响，并且发现这两种抗菌肽的表达水平不同。发现蜂防御素的浓度与基因表达之间存在正相关。证明了给蜜蜂喂食不同蛋白质饲料的重要性以及花粉营养对蜜蜂免疫力的重要性。
