Kim Yeon Gyeong, Hwang Jihye, Choi Hwakyung, Lee Sooyeun
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-gu, Daegu 42601, Korea.
Bugok National Hospital, 145 Bugok-ro, Bugok-myeon, Changnyeong-gun, Gyeongsangnam-do 50365, Korea.
Pharmaceutics. 2018 Jul 4;10(3):84. doi: 10.3390/pharmaceutics10030084.
It is critical to assess the severity of alcohol consumption in certain diseases such as alcohol liver disease and alcohol addiction. Ethyl glucuronide (EtG) is a highly stable metabolite of ethanol in hair; thus, it was proposed as a long-term monitoring marker for alcohol consumption. Therefore, an HPLC-MS/MS method for EtG in hair was developed and applied to a clinical setting to assess the relevance of the EtG concentration and/or the Alcohol Use Disorders Identification Test (AUDIT) score to high-risk alcohol consumption. EtG was extracted from 10 mg of hair using water and analyzed using on-line sample purification coupled to HPLC-MS/MS. The diagnostic performances of the EtG concentration and/or the AUDIT score for detecting high-risk alcohol consumption were statistically evaluated between alcohol addicts ( = 44) and average alcohol users ( = 19). The on-line sample purification resulted in labor-saving with smaller sample amount. Both the EtG concentrations (4.0⁻587.4 pg/mg vs. 12.9⁻74.9 pg/mg) and the AUDIT scores (4⁻40 vs. 5⁻28) obtained from the alcohol addicts were significantly higher than those from the average alcohol users. The performance evaluation demonstrated that the integration score of the EtG concentration and the AUDIT score increased diagnostic performance for high-risk alcohol consumption.
在某些疾病如酒精性肝病和酒精成瘾中，评估酒精摄入量的严重程度至关重要。乙基葡萄糖醛酸苷（EtG）是毛发中乙醇的一种高度稳定的代谢产物；因此，它被提议作为酒精摄入的长期监测标志物。因此，开发了一种用于毛发中EtG的高效液相色谱-串联质谱（HPLC-MS/MS）方法，并将其应用于临床环境，以评估EtG浓度和/或酒精使用障碍识别测试（AUDIT）分数与高风险酒精消费的相关性。使用水从10mg毛发中提取EtG，并使用在线样品净化结合HPLC-MS/MS进行分析。在酒精成瘾者（n = 44）和普通饮酒者（n = 19）之间，对EtG浓度和/或AUDIT分数检测高风险酒精消费的诊断性能进行了统计学评估。在线样品净化节省了劳动力，且样品量更小。酒精成瘾者的EtG浓度（4.0⁻587.4 pg/mg对12.9⁻74.9 pg/mg）和AUDIT分数（4⁻40对5⁻28）均显著高于普通饮酒者。性能评估表明，EtG浓度和AUDIT分数的综合评分提高了高风险酒精消费的诊断性能。
