Department of Biotechnology, College of Engineering, Daegu University, 201 Daegudae-ro, Jillyang, Gyeongsan, Gyeongbuk, 38453, Republic of Korea.
Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 61186, Republic of Korea.
Sci Rep. 2018 Jul 4;8(1):10130. doi: 10.1038/s41598-018-28497-5.
Mito-TEMPO is a well-known mitochondria-specific superoxide scavenger. However, the effect of Mito-TEMPO on porcine embryo development, to our knowledge, has not been studied yet. In the present study, porcine embryos were classified into two groups (G1 and G2) based on the cytoplasm lipid contents at the zygote stage. The development of blastocysts derived from G2 zygotes was reduced (G2:16.2 ± 7.9% vs G1: 26.5 ± 5.9%; 1.6-fold, p < 0.05) compared to those from G1 zygotes. In G2 embryos, the proportion of TUNEL-positive cells was also higher than that of G1 embryos. Superoxide in G2 embryos was significantly increased compared to that in G1 embryos. Mitochondrial membrane potential and ATP production were lower in G2 embryos than in G1 embryos. Phosphorylation of Drp1 at Ser 616 increased in G1 embryos during the cleavage stages compared to that in the zygote but was not significantly different in G2 embryos. Then, the effects of Mito-TEMPO were investigated in G2 embryos. Blastocyst formation rate (G2: 19.1 ± 5.1% vs G2 + Mito-TEMPO: 28.8 ± 4.0%; 1.5-fold, p < 0.05) and mitochondrial aggregation were recovered after superoxide reduction by Mito-TEMPO treatment. Thus, we showed that Mito-TEMPO improves blastocyst development by superoxide reduction in porcine embryos in vitro.
Mito-TEMPO 是一种众所周知的线粒体特异性超氧阴离子清除剂。然而，据我们所知，Mito-TEMPO 对猪胚胎发育的影响尚未得到研究。在本研究中，根据合子阶段的细胞质脂质含量，将猪胚胎分为两组（G1 和 G2）。与 G1 合子来源的囊胚相比，G2 合子来源的囊胚发育减少（G2：16.2±7.9%比 G1：26.5±5.9%；1.6 倍，p<0.05）。在 G2 胚胎中，TUNEL 阳性细胞的比例也高于 G1 胚胎。与 G1 胚胎相比，G2 胚胎中的超氧阴离子明显增加。G2 胚胎中的线粒体膜电位和 ATP 产生低于 G1 胚胎。与合子相比，G1 胚胎在卵裂阶段 Drp1 丝氨酸 616 的磷酸化增加，但 G2 胚胎中没有明显差异。然后，在 G2 胚胎中研究了 Mito-TEMPO 的作用。囊胚形成率（G2：19.1±5.1%比 G2+Mito-TEMPO：28.8±4.0%；1.5 倍，p<0.05）和线粒体聚集在 Mito-TEMPO 处理减少超氧阴离子后得到恢复。因此，我们表明 Mito-TEMPO 通过还原超氧阴离子改善了猪胚胎体外的囊胚发育。
