Mackey Melora, Kurosky Alexander, Robb E Jane, Nazar Ross N
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G2W1, Canada.
Department of Biochemistry and Molecular Biology, University of Texas, Medical Branch, Galveston, TX, 77555, USA.
Mol Biotechnol. 2018 Sep;60(9):665-669. doi: 10.1007/s12033-018-0101-8.
Grafting vegetables for disease resistance has increased greatly in popularity over the past 10 years. Verticillium wilt of tomato is commonly controlled through grafting of commercial varieties on resistant rootstocks expressing the Ve1 R-gene. To mimic the grafted plant, proteomic analyses in tomato were used to identify a suitable root-specific promoter (TMVi), which was used to express the Ve1-allele in susceptible Craigella (Cs) tomato plants. The results indicate that when infected with Verticillim dahliae, race 1, the transformed plants are comparable to resistant cultivars (Cr) or grafted plants.
在过去10年里，用于抗病的蔬菜嫁接技术越来越受欢迎。番茄黄萎病通常通过将商业品种嫁接到表达Ve1抗性基因的抗性砧木上来控制。为了模拟嫁接植物，利用番茄的蛋白质组学分析来鉴定一个合适的根特异性启动子（TMVi），该启动子用于在感病的克雷格拉（Cs）番茄植株中表达Ve1等位基因。结果表明，当用大丽轮枝菌1号小种感染时，转基因植株与抗性品种（Cr）或嫁接植株相当。
