Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, 77555, USA.
Planta. 2019 Jun;249(6):1779-1797. doi: 10.1007/s00425-019-03121-6. Epub 2019 Mar 5.
In tomato, Ve1 gene expression is induced specifically by physical damage or plant wounding, resulting in a defense/stress cascade that mimics responses during Verticillium colonization and wilt. In tomato, Verticillium resistance is determined by the Ve gene locus, which encodes two leucine-rich repeat-receptor-like proteins (Ve1, Ve2); the Ve1 gene is induced differentially while Ve2 is constitutively expressed throughout disease development. These profiles have been observed even during compatible Verticillium interactions, colonization by some bacterial pathogens, and growth of transgenic tomato plants expressing the fungal Ave1 effector, suggesting broader roles in disease and/or stress. Here, we have examined further Ve gene expression in resistant and susceptible plants under abiotic stress, including a water deficit, salinity and physical damage. Using both quantitative RT-PCR and label-free LC-MS methods, changes have been evaluated at both the mRNA and protein levels. The results indicate that Ve1 gene expression responds specifically to physical damage or plant wounding, resulting in a defense/stress cascade that resembles observations during Verticillium colonization. In addition, the elimination or reduction of Ve1 or Ve2 gene function also result in proteomic responses that occur with wilt pathogen and continue to be consistent with an antagonistic relationship between the two genes. Mutational analyses also indicate the plant wounding hormone, systemin, is not required, while jasmonic acid again appears to play a direct role in induction of the Ve1 gene.
在番茄中，Ve1 基因的表达被物理损伤或植物创伤特异性诱导，导致防御/应激级联反应，模拟了Verticillium 定殖和萎蔫期间的反应。在番茄中，Verticillium 抗性由 Ve 基因座决定，该基因座编码两个富含亮氨酸的重复受体样蛋白（Ve1、Ve2）；Ve1 基因在疾病发展过程中差异诱导，而 Ve2 则持续组成性表达。即使在相容的 Verticillium 相互作用、某些细菌病原体的定殖以及表达真菌 Ave1 效应子的转基因番茄植物的生长过程中也观察到了这些特征，表明它们在疾病和/或应激中具有更广泛的作用。在这里，我们在非生物胁迫下，包括水分亏缺、盐度和物理损伤下，进一步研究了抗性和敏感植物中的 Ve 基因表达。使用定量 RT-PCR 和无标记 LC-MS 方法，评估了 mRNA 和蛋白质水平的变化。结果表明，Ve1 基因的表达对物理损伤或植物创伤特异性反应，导致防御/应激级联反应，类似于 Verticillium 定殖期间的观察结果。此外，Ve1 或 Ve2 基因功能的消除或减少也会导致蛋白质组学反应，这些反应与萎蔫病原体有关，并继续与这两个基因之间的拮抗关系一致。突变分析还表明，植物创伤激素系统素不是必需的，而茉莉酸似乎再次在 Ve1 基因的诱导中发挥直接作用。
