Nazar Ross N, Xu Xin, Blaya Fernandez Josefa, Shittu Hakeem, Kurosky Alexander, Robb Jane
a Department of Molecular and Cellular Biology , University of Guelph , Guelph , ON Canada.
b Department of Biochemistry and Molecular Biology , University of Texas , Galveston , TX USA.
Plant Signal Behav. 2018;13(6):e1475807. doi: 10.1080/15592324.2018.1475807. Epub 2018 Jun 25.
In tomato the Ve1-gene provides resistance to the vascular pathogen, Verticillium dahliae, race 1; ve1 plants are susceptible. Reciprocal grafts of resistant and susceptible tomato near-isolines were used to examine proteomic changes and, in particular, the effect of the Ve1-gene on the defence/stress protein cascade induced during Verticillium wilt. Based on label-free LC-MS, the results indicate that this defence response is cell-specific, correlates with overall fungal colonization and is mitigated by Ve1 function. The influence of the Ve1-gene in resistant tissues, however, is not actually transferred to susceptible tissues in the grafted plant.
在番茄中，Ve1基因赋予对维管束病原体大丽轮枝菌1号小种的抗性；ve1基因的植株易感病。利用抗性和易感番茄近等基因系的互作嫁接来检测蛋白质组变化，尤其是Ve1基因对黄萎病期间诱导的防御/应激蛋白级联反应的影响。基于无标记液相色谱-质谱分析，结果表明这种防御反应具有细胞特异性，与真菌的整体定殖相关，并因Ve1功能而减轻。然而，Ve1基因在抗性组织中的影响实际上并未转移到嫁接植株中的易感组织。
