Kaneko Tetsuya, Fujioka Takashi, Suzuki Yosuke, Nagano Toshiaki, Sato Yuhki, Asakura Syunji, Itoh Hiroki
Department of Clinical Pharmacy, Oita University Hospital, Oita, Japan.
Department of Pharmacy, Oita Red Cross Hospital, Oita, Japan.
J Clin Lab Anal. 2018 Nov;32(9):e22587. doi: 10.1002/jcla.22587. Epub 2018 Jul 5.
Different measured values for tacrolimus were obtained with different automated immunoassays. We aimed to examine the differences in the blood tacrolimus concentrations measured by the major immunoassay systems commercially available in Japan.
Whole-blood samples from 118 patients were assayed by 3 commercial assays: chemiluminescent enzyme immunoassay (CLIA), affinity column-mediated immunoassay (ACMIA), and enzyme-multiplied immunoassay technique (EMIT). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for reference.
The correlation coefficient of immunoassay vs LC-MS/MS was excellent for ACMIA (.83) and CLIA (.81) and good for EMIT (.71). The mean error was negative for ACMIA and positive for CLIA and EMIT. The mean absolute error and root-mean-square error were almost the same for ACMIA and CLIA and lower than those for EMIT.
The ACMIA and CLIA yield considerably better results than the EMIT for monitoring blood tacrolimus concentrations.
不同的自动化免疫分析方法测得的他克莫司值不同。我们旨在研究日本市售主要免疫分析系统所测血液中他克莫司浓度的差异。
采用3种商业检测方法对118例患者的全血样本进行检测：化学发光酶免疫分析（CLIA）、亲和柱介导免疫分析（ACMIA）和酶放大免疫分析技术（EMIT）。液相色谱-串联质谱法（LC-MS/MS）用作参考。
免疫分析与LC-MS/MS的相关系数，ACMIA为0.83，CLIA为0.81，两者极佳；EMIT为0.71，良好。ACMIA的平均误差为负，CLIA和EMIT的平均误差为正。ACMIA和CLIA的平均绝对误差和均方根误差几乎相同，且低于EMIT。
在监测血液他克莫司浓度方面，ACMIA和CLIA比EMIT的结果要好得多。
