利用热酸消解和基质辅助激光解吸电离飞行时间质谱法对蓖麻毒素进行快速分析。
Rapid analysis of ricin using hot acid digestion and MALDI-TOF mass spectrometry.
作者信息
Chen Dapeng, Bryden Wayne A, Fenselau Catherine
机构信息
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
Zeteo Tech Inc., Sykesville, MD, USA.
出版信息
J Mass Spectrom. 2018 Oct;53(10):1013-1017. doi: 10.1002/jms.4257. Epub 2018 Aug 15.
Abstract
Ricin is a protein toxin of considerable interest in forensics. A novel strategy is reported here for rapid detection of ricin based on microwave-assisted hot acid digestion and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. Ricin samples are subjected to aspartate-selective hydrolysis, and biomarker peptide products are characterized by mass spectrometry. Spectra are obtained using post source decay and searched against a protein database. Several advantages are offered by chemical hydrolysis, relative to enzymatic hydrolysis, notably speed, robustness, and the production of heavier biomarkers. Agglutinin contamination is reliably recognized, as is the disulfide bond strongly characteristic of ricin.
摘要
蓖麻毒素是一种在法医学领域备受关注的蛋白质毒素。本文报道了一种基于微波辅助热酸消解和基质辅助激光解吸/电离飞行时间质谱的蓖麻毒素快速检测新策略。蓖麻毒素样品进行天冬氨酸选择性水解，生物标志物肽产物通过质谱进行表征。使用源后衰变获得光谱，并与蛋白质数据库进行比对搜索。相对于酶促水解，化学水解具有几个优点，特别是速度快、稳定性强以及能产生分子量更大的生物标志物。凝集素污染能够被可靠识别，蓖麻毒素特有的二硫键也能被识别。
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