Chen Dapeng, Bryden Wayne A, Fenselau Catherine
Department of Chemistry and Biochemistry, University of Maryland, College Park, MD, USA.
Zeteo Tech Inc, Sykesville, MD, USA.
Int J Mass Spectrom. 2019 Jan;435:227-233. doi: 10.1016/j.ijms.2018.10.033. Epub 2018 Nov 2.
Rapid identification of spores in the environment has depended primarily on a family of small acid soluble proteins (SASPs) as biomarkers. However, SASP sequences and molecular masses are similar or identical in some critical cases. For example, some strains of , and cannot be distinguished from strains of based on SASPs. Consequently, additional or alternative biomarkers should be sought. In this study microwave-assisted hot acid hydrolysis was coupled with mass spectrometry as a potentially powerful approach to the rapid automatable characterization of spores. Hot acid provides lysis of the spores, Asp-selective hydrolysis of proteins, and peptides compatible with automated analysis of either peptide fingerprints or tandem mass spectra. Peptide biomarkers are compared here for a selection of spores, and peptides unique to each spore type are identified.
环境中孢子的快速鉴定主要依赖于一类小的酸溶性蛋白（SASP）作为生物标志物。然而，在一些关键情况下，SASP序列和分子量相似或相同。例如，基于SASP无法区分某些芽孢杆菌属、梭菌属和脱硫肠状菌属的菌株与芽孢八叠球菌属的菌株。因此，应寻找额外的或替代的生物标志物。在本研究中，微波辅助热酸水解与质谱联用，作为一种潜在的强大方法用于芽孢八叠球菌属孢子的快速自动化表征。热酸可使孢子裂解、对蛋白质进行天冬氨酸选择性水解，并产生与肽指纹或串联质谱自动分析兼容的肽段。本文比较了一系列芽孢八叠球菌属孢子的肽生物标志物，并鉴定了每种孢子类型特有的肽段。
