Fidan Mehmet, Erez Mehmet Emre, İnal Behcet, Pinar Süleyman Mesut, Altintaş Serdar
Biology Department, Science and Art Faculty, Siirt University, Siirt, Turkey.
Agricultural Biotechnology Department, Agriculture Faculty, Siirt University, Siirt, Turkey.
Cell Mol Biol (Noisy-le-grand). 2018 May 30;64(7):14-18.
The quality of grape cultivars not only depends on the grape cultivar but also is influenced by the molecular concepts and agro-climatic factors. For this purpose, four different grape cultivars were collected from five different locations in Siirt province (Turkey). Totally twenty different grape cultivars were investigated. In the present study, the antioxidant activity (total phenolic, flavonoid, proanthocyanidin content, DPPH and FRAP activity) in seeds were indicated and phylogenetic analysis (cpDNA;trnL-F region) of twenty native grape cultivars were investigated to construct their phylogenetic tree. According to reported data on antioxidant activity and content of phytochemicals, all cultivars exhibited different values from each other, but Rutik and Gadüv cultivars were found as significantly higher in comparison to others. According to bioinformatics analysis, twenty grape cultivars were distributed into six different major groups. Rutik and Sevkeye cultivars exhibit significant distinction from other grape cultivars. The phylogenetic analysis was also associated and supported with the results of obtained data from bioactivity. The bioactivity and phylogenetic analysis were firstly identified and quantified in these grape cultivars, however, with regard to obtained data from the current study, the grape cultivars grown in Siirt province were indicated significant and valuable results and as a result, these cultivars have to be evaluated before extinction.
葡萄品种的品质不仅取决于葡萄品种本身，还受到分子概念和农业气候因素的影响。为此，从土耳其锡尔特省的五个不同地点收集了四个不同的葡萄品种。总共对二十个不同的葡萄品种进行了研究。在本研究中，测定了种子中的抗氧化活性（总酚、黄酮、原花青素含量、DPPH和FRAP活性），并对二十个本地葡萄品种进行了系统发育分析（叶绿体DNA；trnL-F区域）以构建它们的系统发育树。根据已报道的抗氧化活性和植物化学物质含量的数据，所有品种之间呈现出不同的值，但发现鲁蒂克（Rutik）和加杜夫（Gadüv）品种相比其他品种显著更高。根据生物信息学分析，二十个葡萄品种被分为六个不同的主要组。鲁蒂克和塞夫凯耶（Sevkeye）品种与其他葡萄品种表现出显著差异。系统发育分析也与生物活性获得的数据结果相关并得到支持。这些葡萄品种的生物活性和系统发育分析首次得到鉴定和量化，然而，就本研究获得的数据而言，生长在锡尔特省的葡萄品种显示出显著且有价值的结果，因此，这些品种在灭绝之前必须进行评估。
