不同激素组合对千日红愈伤组织再生的影响

Effect of different hormonal combinations on regeneration of callus of Gomphrena globosa L.

作者信息

Ghaffar Iram, Ali Basharat, Hasnain Shahida

机构信息

Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan.

出版信息

Pak J Biol Sci. 2007 Oct 15;10(20):3708-12. doi: 10.3923/pjbs.2007.3708.3712.

DOI:10.3923/pjbs.2007.3708.3712

PMID:19093487

Abstract

The main aim of this study was to observe the effect of different hormonal combinations on regeneration of callus of Gomphrena globosa L. For this purpose callus was obtained from seeds G. globosa inoculated on MS medium supplemented with 4 mg L(-1) 2, 4-D and 10% coconut milk. After callus formation callus was inoculated on Murashige and Skoog's medium supplemented with different combinations of BAP, NAA and GA3 to observe different responses such as regeneration, callus friability, callus proliferation and pigmentation. In BAP and NAA root regeneration was observed at 1.5 mg L(-1) BAP+1 mg L(-1) NAA whereas rest of the combinations showed callus proliferation. In BAP and GA3, root regeneration was observed in most of the combinations and some combinations also showed shoot induction. Shoot regeneration was observed on 0.5 mg L(-1) BAP + 7 mg L(-1) GA3 and 1 mg L(-1) BAP + 0.2 mg L(-1) GA3. The effect of all these combinations on auxin, acid phosphatase and soluble protein content was also observed.

摘要

本研究的主要目的是观察不同激素组合对千日红愈伤组织再生的影响。为此，从接种于添加4 mg L(-1) 2,4 - D和10%椰乳的MS培养基上的千日红种子中获得愈伤组织。愈伤组织形成后，将其接种于添加不同组合的BAP、NAA和GA3的Murashige和Skoog培养基上，以观察不同的反应，如再生、愈伤组织脆性、愈伤组织增殖和色素沉着。在BAP和NAA组合中，在1.5 mg L(-1) BAP + 1 mg L(-1) NAA时观察到根再生，而其他组合显示愈伤组织增殖。在BAP和GA3组合中，大多数组合观察到根再生，一些组合也显示出芽诱导。在0.5 mg L(-1) BAP + 7 mg L(-1) GA3和1 mg L(-1) BAP + 0.2 mg L(-1) GA3时观察到芽再生。还观察了所有这些组合对生长素、酸性磷酸酶和可溶性蛋白质含量的影响。