Department of Oral and Maxillofacial Pathology, Tamil Nadu Government Dental College and Hospital (Affiliated to The Tamil Nadu Dr. MGR Medical University, No. 69, Anna Salai, Guindy, Chennai 600 032, Tamil Nadu, India), Chennai, India.
Oral Dis. 2018 Nov;24(8):1538-1544. doi: 10.1111/odi.12934. Epub 2018 Aug 14.
To analyse the immunohistochemical expression of ameloblastin in the bell stage of tooth germ and compare with ameloblastoma to determine the level of differentiation of tumour cells.
This study included eleven human tooth germs with four in the early and seven in the late bell stage, and six selected archival tissue samples of ameloblastomas were studied using haematoxylin and eosin, Masson's trichrome and ameloblastin.
All eleven tooth germs reacted positively to ameloblastin with a characteristic inverted and sequential pattern of expression in the acellular zone of the dental papilla and enamel organ. Of the six cases of ameloblastoma, five cases showed a variable level of expression of ameloblastin in the tumour cells, whereas in one case, ameloblastin was negative in the tumour cells but positive in the stromal fibrous tissue collar.
Expression of ameloblastin in human tooth germ is related to differentiation and mineralization, and it correlates with the state of differentiation of the tumour cells in ameloblastoma.
分析钟状期牙胚中釉原蛋白的免疫组织化学表达，并与成釉细胞瘤进行比较，以确定肿瘤细胞的分化程度。
本研究包括 11 个人类牙胚，其中 4 个处于早期钟状期，7 个处于晚期钟状期，并使用苏木精和伊红、马松三色染色和釉原蛋白对 6 个选定的成釉细胞瘤存档组织样本进行了研究。
所有 11 个牙胚均对釉原蛋白呈阳性反应，在牙髓和釉器的无细胞区呈特征性的倒置和顺序表达模式。在 6 例成釉细胞瘤中，5 例肿瘤细胞中釉原蛋白表达水平不同，而在 1 例中，肿瘤细胞中釉原蛋白阴性，但间质纤维组织环阳性。
釉原蛋白在人牙胚中的表达与分化和矿化有关，并与成釉细胞瘤中肿瘤细胞的分化状态相关。
