School of Food Science and Technology , Jiangnan University , 1800 Lihu Avenue , Wuxi , Jiangsu 214122 , China.
Jiangsu Institute of Nuclear Medicine , Key Laboratory of Nuclear Medicine, Ministry of Health , 20 Qian Rong , Wuxi , Jiangsu 214063 , China.
J Agric Food Chem. 2018 Jul 25;66(29):7655-7662. doi: 10.1021/acs.jafc.8b02012. Epub 2018 Jul 16.
The objective of our research was to evaluate the molecular mechanism of the anti-inflammatory effects of surface-layer protein (Slp) derived from Lactobacillus acidophilus NCFM in lipopolysaccharide-induced RAW264.7 cells. Our results presented that Slp, with an apparent size of 46 kDa, attenuated the production of TNF-α, IL-1β, and reactive oxygen species (ROS), by inhibiting the MAPK and NF-κB signaling pathways. In addition, 10 μg mL of Slp significantly inhibited NO and PGE production ( P < 0.001) through downregulating the expression levels of iNOS and COX-2 protein. Furthermore, Slp was found to inhibit NF-κB p65 translocation into the nucleus to activate inflammatory gene transcription. These findings suggest that Slp is a potential immune-modulating bioactive protein derived from probiotics and holds promise for use as an additive in functional foods.
我们的研究目的是评估嗜酸乳杆菌 NCFM 表面层蛋白（Slp）的抗炎作用的分子机制。在脂多糖诱导的 RAW264.7 细胞中，我们的结果表明，Slp 通过抑制 MAPK 和 NF-κB 信号通路，减轻 TNF-α、IL-1β 和活性氧（ROS）的产生。此外，10μg/mL 的 Slp 通过下调 iNOS 和 COX-2 蛋白的表达水平，显著抑制 NO 和 PGE 的产生（P<0.001）。此外，Slp 被发现抑制 NF-κB p65 向核内易位以激活炎症基因转录。这些发现表明 Slp 是一种潜在的免疫调节益生菌来源的生物活性蛋白，有望作为功能性食品的添加剂。
