Morgan J H, Papas T S, Parsons J T
J Natl Cancer Inst. 1985 Nov;75(5):937-47. doi: 10.1093/jnci/75.5.937.
The myc gene has been implicated in the genesis of various neoplasms in birds, mice, and humans and was originally identified as the cellular homologue of the transforming gene (v-myc) of the avian myelocytomatosis virus MC29. For specific antisera to be obtained for the myc gene product, a bacterial expression vector was constructed in which the coding sequences for approximately 20 kd of MC29 p110gag-myc (amino acid residues 502 to 678) were placed between the coding sequences for the amino terminal 13 kd of Rous sarcoma virus pp60src and the coding sequences for 112 kd of beta-galactosidase. Expression of this tripartite gene was driven by a hybrid trp-lac promoter under lac repressor control. Induction of expression resulted in the production of a 145-kd hybrid protein containing src, myc, and beta-galactosidase sequences. The hybrid protein was purified and injected into rabbits to produce antisera. The resultant antisera immunoprecipitated p110gag-myc and p58myc -p60myc from MC29- and MH2-infected nonproducer quail fibroblasts, respectively. In addition, the antisera also immunoprecipitated a 58-kd protein from the bursal lymphoma cell line BK25, which was identified as chicken c (cellular)-myc gene product.
myc基因与鸟类、小鼠和人类的各种肿瘤发生有关，最初被鉴定为禽骨髓细胞瘤病毒MC29的转化基因（v-myc）的细胞同源物。为了获得针对myc基因产物的特异性抗血清，构建了一种细菌表达载体，其中约20kd的MC29 p110gag-myc（氨基酸残基502至678）的编码序列置于劳氏肉瘤病毒pp60src的氨基末端13kd的编码序列与112kd的β-半乳糖苷酶的编码序列之间。这个三方基因的表达由乳糖阻遏物控制下的杂合trp-lac启动子驱动。诱导表达导致产生一种包含src、myc和β-半乳糖苷酶序列的145kd杂合蛋白。该杂合蛋白被纯化并注射到兔子体内以产生抗血清。所得抗血清分别从感染MC29和MH2的非生产性鹌鹑成纤维细胞中免疫沉淀p110gag-myc和p58myc-p60myc。此外，该抗血清还从法氏囊淋巴瘤细胞系BK25中免疫沉淀出一种58kd的蛋白，该蛋白被鉴定为鸡c（细胞）-myc基因产物。
