Lautenberger J A, Court D, Papas T S
Gene. 1983 Jul;23(1):75-84. doi: 10.1016/0378-1119(83)90218-4.
A plasmid, pJL6, was constructed that contains a unique ClaI site twelve codons beyond the bacteriophage lambda cII gene initiation codon. This site allowed us to fuse the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc gene to the amino-terminal portion of the cII gene. Transcription of the hybrid gene is controlled from the phage lambda pL promoter. When this promoter is derepressed, Escherichia coli cells harboring the chimeric plasmid produce a level of cII-myc fusion protein greater than 5% of total cellular protein. Antibodies raised by this protein immunoprecipitate the MC29 gag-myc gene product, P110gag-myc.
构建了一种质粒pJL6，它在噬菌体λ cII基因起始密码子下游十二个密码子处含有一个独特的ClaI位点。该位点使我们能够将禽成髓细胞瘤病毒（MC29）v-myc基因的羧基末端序列与cII基因的氨基末端部分融合。杂合基因的转录由噬菌体λ pL启动子控制。当该启动子去阻遏时，携带嵌合质粒的大肠杆菌细胞产生的cII-myc融合蛋白水平超过细胞总蛋白的5%。由该蛋白产生的抗体可免疫沉淀MC29 gag-myc基因产物P110gag-myc。
