Faculty of Veterinary, Siirt University, Siirt, Turkey.
Health Services Vocational School, Bingol University, Bingol, Turkey.
J Biochem Mol Toxicol. 2018 Aug;32(8):e22170. doi: 10.1002/jbt.22170. Epub 2018 Jul 5.
In this study, the effects of astaxanthin (AST) that belongs to carotenoid family and cadmium (Cd), which is an important heavy metal, on rat erythrocyte G6PD, 6PGD, GR, and TrxR enzyme activities in vivo and on rat erythrocyte 6PGD enzyme activity in vitro were studied. In in vitro studies, 6PGD enzyme was purified from rat erythrocytes with 2',5'-ADP Sepharose4B affinity chromatography. Results showed inhibition of enzyme by Cd at IC ; 346.5 μM value and increase of 6PGD enzyme activity by AST. In vivo studies showed an increase in G6PD, 6PGD, and GR enzyme activities (P ˃ 0.05) and no chance in TrxR enzyme activity by AST. Cd ion inhibited G6PD, 6PGD, and GR enzyme activities (P ˂ 0.05) and also decreased TrxR enzyme activity (P ˃ 0.05). AST + Cd group G6PD enzyme activity was statistically low compared with control group (P ˂ 0.05). 6PGD and TrxR enzyme activities decreased without statistical significance (P ˃ 0.05); however, GR enzyme activity increased statistically significantly (P ˂ 0.05).
在这项研究中，研究了属于类胡萝卜素家族的虾青素（AST）和镉（Cd）对体内大鼠红细胞 G6PD、6PGD、GR 和 TrxR 酶活性以及体外大鼠红细胞 6PGD 酶活性的影响。在体外研究中，用 2'，5'-ADP Sepharose4B 亲和层析法从大鼠红细胞中纯化 6PGD 酶。结果表明，Cd 在 IC ； 346.5 μM 值时抑制酶，AST 增加 6PGD 酶活性。体内研究表明，AST 增加 G6PD、6PGD 和 GR 酶活性（P ˃ 0.05），但 TrxR 酶活性没有变化。Cd 离子抑制 G6PD、6PGD 和 GR 酶活性（P ˂ 0.05），并降低 TrxR 酶活性（P ˃ 0.05）。AST + Cd 组 G6PD 酶活性与对照组相比统计学降低（P ˂ 0.05）。6PGD 和 TrxR 酶活性降低，但无统计学意义（P ˃ 0.05）；然而，GR 酶活性统计学显著增加（P ˂ 0.05）。
