Bio-Organic Chemistry research group, Institute for Complex Molecular Systems, Eindhoven University of Technology, PO Box 513 (STO3.41), 5600 MB, Eindhoven, The Netherlands.
Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands.
Chem Asian J. 2018 Nov 16;13(22):3518-3525. doi: 10.1002/asia.201800842. Epub 2018 Aug 14.
Capsids of the cowpea chlorotic mottle virus (CCMV) hold great promise for use as nanocarriers in vivo. A major drawback, however, is the lack of stability of the empty wild-type virus particles under physiological conditions. Herein, the assembly behavior and stability under nearly physiological conditions of protein-based block copolymers composed of the CCMV capsid protein and two hydrophobic elastin-like polypeptides are reported. UV/Vis spectroscopy studies, dynamic light-scattering analysis, and TEM measurements demonstrate that both hybrid variants form stable capsids at pH 7.5, physiological NaCl concentration, and 37 °C. The more hydrophobic variant also remains stable in a cell culture medium. These engineered, hybrid CCMV capsid particles can therefore be regarded as suitable candidates for in vivo applications.
豇豆花叶病毒（CCMV）的衣壳在体内用作纳米载体具有很大的应用前景。然而，主要缺点是在生理条件下，空的野生型病毒颗粒缺乏稳定性。本文报道了由 CCMV 衣壳蛋白和两种疏水性弹性蛋白样多肽组成的基于蛋白质的嵌段共聚物在近生理条件下的组装行为和稳定性。紫外/可见光谱研究、动态光散射分析和 TEM 测量表明，两种杂交变体在 pH 值为 7.5、生理氯化钠浓度和 37°C 下均能形成稳定的衣壳。疏水性更强的变体在细胞培养基中也保持稳定。因此，这些工程化的、杂交的 CCMV 衣壳颗粒可以被视为体内应用的合适候选物。
