Müller Maria Isabel, Morais Drausio Honorio, Silva Reinaldo José da
Universidade Estadual Paulista "Júlio de Mesquita Filho", Instituto de Biociências, Departamento de Parasitologia, Distrito de Rubião Júnior s/n, CEP 18618-689, Botucatu, São Paulo, Brazil.
Universidade Federal Rural da Amazônia (UFRA), PA 275, Km 13, Zona Rural. CEP: 68.515-000, Parauapebas, Pará, Brazil.
Acta Parasitol. 2018 Sep 25;63(3):522-526. doi: 10.1515/ap-2018-0062.
Three valid species of Haplometroides Odhner, 1910 parasitise snakes and amphisbaenians from South America. This study provides additional data on morphometric and molecular phylogenetic position inferred from the nuclear ribosomal gene 28S (partial). DNA sequences were isolated from Haplometroides intercaecalis Silva, Ferreira and Strüssmann, 2007 found in one specimen of Phalotris matogrossensis Lema, D'Agostini and Cappellari, 2005. Five digenean specimens were recovered from the esophagus of this snake, and four specimens were used for morphometrical studies and one specimen for molecular analysis. Phylogenetic analysis using maximum likelihood and Bayesian methods was conducted with sequences available for the order Plagiorchiida and its phylogenetic position places H. intercaecalis among the brachycoeliids Brachycoelium (Dujardin, 1845) Stiles and Hassall, 1898 and Parabrachycoelium Pérez-Ponce de León, Mendoza-Garfias, Razo-Mendivil and Parra-Olea, 2011, and the mesocoeliid Mesocoelium Odhner, 1910, not closely related to plagiorchids as expected. Due to morphological differences among these families, it may be necessary to create a new family to accommodate Haplometroides spp. However, more genera/taxa as well as other molecular markers should be added in future studies to confirm our results and resolve this matter. This is the first phylogenetic positioning of digeneans of the genus Haplometroides, contributing to the systematic analysis of the helminthological biodiversity of Neotropical snakes.
1910年命名的哈氏属（Haplometroides Odhner）中有三个有效物种寄生于南美洲的蛇类和蚓蜥类。本研究提供了关于从核糖体基因28S（部分序列）推断出的形态测量和分子系统发育位置的更多数据。从2007年发现于2005年命名的马托格罗索叶鼻蛇（Phalotris matogrossensis Lema, D'Agostini and Cappellari）的一个标本中的肠间哈氏吸虫（Haplometroides intercaecalis Silva, Ferreira and Strüssmann）中分离出DNA序列。从这条蛇的食管中采集到五只复殖吸虫标本，其中四只用于形态测量研究，一只用于分子分析。使用最大似然法和贝叶斯方法，对斜睾目（Plagiorchiida）可得序列进行了系统发育分析，其系统发育位置将肠间哈氏吸虫置于短肠科（Brachycoeliidae）的短肠属（Brachycoelium (Dujardin, 1845) Stiles and Hassall, 1898）和副短肠属（Parabrachycoelium Pérez-Ponce de León, Mendoza-Garfias, Razo-Mendivil and Parra-Olea, 2011）以及中肠科（Mesocoeliidae）的中肠属（Mesocoelium Odhner, 19,10）之中，并不像预期的那样与斜睾科关系密切。由于这些科之间存在形态差异，可能有必要创建一个新科来容纳哈氏属物种。然而，未来的研究应增加更多的属/分类单元以及其他分子标记，以证实我们的结果并解决这个问题。这是哈氏属复殖吸虫的首次系统发育定位，有助于对新热带区蛇类的蠕虫生物多样性进行系统分析。
