Institute of Biosciences, Section of Parasitology, São Paulo State University (UNESP), Rua Professor Doutor Antônio Celso Wagner Zanin, 250, Botucatu, São Paulo, 18618-689, Brazil.
Department of Biological Sciences, Federal University of São Paulo (UNIFESP), Rua Professor Arthur Riedel, 275, Jardim Eldorado, Diadema, São Paulo, 09972-270, Brazil.
Parasitol Res. 2021 Mar;120(3):971-977. doi: 10.1007/s00436-020-07041-3. Epub 2021 Jan 6.
Two digenean species, Infidum infidum Faria, 1910 (Dicrocoeliidae) and Travtrema stenocotyle Cohn, 1902 (Plagiorchiidae), were collected in the large pit viper Bothrops moojeni Hoge, 1966 from Reserva Particular do Patrimônio Natural Cisalpina, municipality of Brasilândia, Mato Grosso do Sul State, Brazil. In this study, we provide the first molecular characterisation using the 28S rDNA and phylogenetic position data of these two common digeneans from B. moojeni. The molecular framework revealed topologies with strongly supported clades using maximum likelihood and Bayesian inference methods, positioned I. infidum among Plagiorchiidae and not among Dicrocoeliidae as expected and T. stenocotyle (Plagiorchiidae) surprisingly grouped as a sister group to Allassogonoporidae, Microphallidae, Pleurogenidae, and Prosthogonimidae, not related to plagiorchids. Our molecular phylogenetic data showed that these species may not correspond to their assigned families and encourage future studies on the systematic of these understudied groups.
两种复殖吸虫，即 Infidum infidum Faria, 1910（双腔科）和 Travtrema stenocotyle Cohn, 1902（棘口科），从巴西马托格罗索州 Brasilândia 市 Cisalpina 自然遗产保护区的大型坑蝮 Bothrops moojeni Hoge, 1966 中采集。在这项研究中，我们使用 28S rDNA 对这两种常见的复殖吸虫进行了首次分子特征描述，并提供了系统发育位置数据。分子框架使用最大似然法和贝叶斯推断法揭示了具有强烈支持的进化枝的拓扑结构，将 I. infidum 定位在棘口科内，而不是预期的双腔科内，而 T. stenocotyle（棘口科）令人惊讶地与 Allassogonoporidae、Microphallidae、Pleurogenidae 和 Prosthogonimidae 聚为姐妹群，而与棘口科无关。我们的分子系统发育数据表明，这些物种可能与其所属的科并不对应，鼓励对这些研究较少的群体的系统发生进行未来研究。
