Kornacka Aleksandra, Cybulska Aleksandra, Moskwa Bożena
Witold Stefański Institute of Parasitology, Polish Academy of Sciences, Warsaw, Poland.
Acta Parasitol. 2018 Sep 25;63(3):634-639. doi: 10.1515/ap-2018-0072.
Toxoplasma gondii, a coccidian parasite known to infect almost all warm-blooded animals, is the cause of one of the most common zoonotic parasitic diseases. The aim of the study is to determine whether the 529 bp fragment or the TGR1E gene is more useful target for PCR identification of T. gondii, for common use. The brains of 221 carnivores and omnivores collected between 2013 and 2015 from north-eastern Poland were examined for the presence of this parasite. The DNA was extracted and then amplified using specific primers. Positive results were obtained in 24% of brain samples using the TGR1E target and 19% using the 529 bp sequence. The results demonstrate that both TGR1E and 529 bp repeat element are suitable for detecting T. gondii DNA in wildlife animals, and the combination of two methods is necessary to obtain reliable results.
刚地弓形虫是一种已知可感染几乎所有温血动物的球虫寄生虫，是最常见的人畜共患寄生虫病之一的病因。本研究的目的是确定529 bp片段或TGR1E基因作为用于常规PCR鉴定刚地弓形虫的更有用靶标。对2013年至2015年期间从波兰东北部采集的221只食肉动物和杂食动物的大脑进行了该寄生虫检测。提取DNA，然后使用特异性引物进行扩增。使用TGR1E靶标在24%的脑样本中获得阳性结果，使用529 bp序列在19%的脑样本中获得阳性结果。结果表明，TGR1E和529 bp重复元件均适用于检测野生动物中的刚地弓形虫DNA，两种方法结合对于获得可靠结果是必要的。
