Baptistiolli Lillian, Narciso Luis Gustavo, Almeida Breno Fernando Martins de, Bosco Anelise Maria, Souza Jucilene Conceição de, Torrecilha Rafaela Beatriz Pintor, Pereira Priscila Préve, Figueiredo Renata Nogueira, Garcia José Fernando, Kaneto Carlos Noriyuki, Ciarlini Paulo César
Department of Veterinary Medicine, Surgery and Animal Reproduction, São Paulo State University, Clóvis Pestana, 793, CEP 16050-680, Araçatuba, São Paulo, Brazil.
Department of Support, Production and Animal Health, São Paulo State University, Clóvis Pestana, 793, CEP 16050-680, Araçatuba, São Paulo, Brazil.
Acta Parasitol. 2018 Sep 25;63(3):504-514. doi: 10.1515/ap-2018-0060.
The mechanisms responsible for the imbalance between oxidants and antioxidants in sheep infected with Haemonchus contortus are not well established. This study aimed to prove the hypothesis that oxidative stress occurring during infection by H. contortus varies according to breed, and that the parasite burden correlates with hypoalbuminaemia and anaemia. Thus, after deworming and confirming the absence of infection, two different sheep breeds, Suffolk (n = 15) and Santa Ines (n = 22), were orally inoculated with a single dose of 5,000 L3 of H. contortus. The egg counts per gram of faeces (EPG), packed cell volume (PCV) and concentrations of several plasma markers of oxidative stress (lipid peroxidation, albumin, uric acid, total bilirubin, total antioxidant capacity [TAC], total oxidant concentration [TOC] and the oxidative stress index [OSI]) were quantified before (control group) and during the experimental infection (28, 34 and 42 days post-inoculation). In both breeds, TOC increased at 28 days and TAC increased at 42 days. In Suffolk sheep, there was a positive correlation of EPG with oxidant components (28 days) and a negative correlation of EPG with PCV (42 days). In Santa Ines sheep, there was a positive correlation of EPG with bilirubin (r = 0.492; p = 0.020). H. contortus infection caused oxidative stress, which varied according to the breed. Parasite burden was not associated with hypoalbuminaemia, whereas there was a negative correlation with PCV. This research provides the first evidence that the antioxidant status contributes more to the resilience to H. contortus in Santa Ines sheep compared to Suffolk sheep.
感染捻转血矛线虫的绵羊体内氧化剂与抗氧化剂失衡的机制尚未完全明确。本研究旨在验证以下假设：捻转血矛线虫感染期间发生的氧化应激因品种而异，且寄生虫负荷与低白蛋白血症和贫血相关。因此，在驱虫并确认无感染后，将两个不同品种的绵羊，萨福克羊（n = 15）和圣伊内斯羊（n = 22），口服接种单剂量5000条捻转血矛线虫的L3期幼虫。在实验感染前（对照组）和感染期间（接种后28、34和42天），对每克粪便中的虫卵数（EPG）、血细胞压积（PCV）以及几种氧化应激血浆标志物（脂质过氧化、白蛋白、尿酸、总胆红素、总抗氧化能力[TAC]、总氧化剂浓度[TOC]和氧化应激指数[OSI]）的浓度进行了定量分析。在两个品种中，TOC在28天时升高，TAC在42天时升高。在萨福克羊中，EPG与氧化剂成分在28天时呈正相关，EPG与PCV在42天时呈负相关。在圣伊内斯羊中，EPG与胆红素呈正相关（r = 0.492；p = 0.02）。捻转血矛线虫感染会引起氧化应激，且因品种而异。寄生虫负荷与低白蛋白血症无关，但与PCV呈负相关。本研究首次证明，与萨福克羊相比，抗氧化状态对圣伊内斯羊抵抗捻转血矛线虫的能力贡献更大。
