de Oliveira Franciele Delevati, Freddo Natália, Zorzi Natalie Renata, Grando Luciana Grazziotin Rossato, Costa Márcio Machado, Tamagno Wagner Antonio, Botelho Vieira Maria Isabel
Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo - UPF, BR 285, Km 292, Passo Fundo, Rio Grande do Sul, 99052-900, Brazil.
Faculdade de Medicina Veterinária - FAMEV, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil.
Trop Anim Health Prod. 2025 Jun 9;57(5):251. doi: 10.1007/s11250-025-04513-5.
The present study aimed to evaluate the impact of natural infection by Haemonchus contortus in pregnant ewes. Clinical assessments were conducted using the FAMACHA method, eggs per gram (EPG), and coproculture. Hematological and biochemical parameters, including cortisol levels, oxidative stress markers, hematocrit (HCT), and total plasma protein (TPP), were also analyzed. A total of 100 sheep naturally infected with H. contortus were used, 50 from the Corriedale breed and 50 from the Ile de France breed. Results indicated an increase in EPG (from 300 to 580 eggs by day 63). H. contortus was the predominant species identified in coproculture. Both breeds showed a proportional reduction in anemia severity as assessed by the FAMACHA© method throughout the experiment. HCT decreased near parturition, reaching 29% in both breeds, but increased by 0.09 units per day postpartum, reflecting physiological adaptations unrelated to parasitism. Hypoproteinemia (6.6 g/dL) was observed during the fourth sampling, coinciding with the rise in EPG. Higher serum cortisol levels were detected in non-anemic ewes (17.84 µg/dL) compared to anemic ones (10.19 µg/dL), particularly in the postpartum period. No significant changes were observed in oxidative stress markers. The study concluded that hypoproteinemia is associated with increased EPG. Even the highest EPG value of 580 eggs was not indicative of a severe parasitic infection. The HCT decreased proportionally between breeds over the course of the study, reaching its lowest value as parturition approached.
本研究旨在评估捻转血矛线虫自然感染对怀孕母羊的影响。采用FAMACHA方法、每克虫卵数（EPG）和粪便培养进行临床评估。还分析了血液学和生化参数，包括皮质醇水平、氧化应激标志物、血细胞比容（HCT）和总血浆蛋白（TPP）。总共使用了100只自然感染捻转血矛线虫的绵羊，其中50只来自考力代品种，50只来自法兰西岛品种。结果表明EPG增加（到第63天时从300个虫卵增加到580个虫卵）。捻转血矛线虫是粪便培养中鉴定出的主要虫种。在整个实验过程中，通过FAMACHA©方法评估，两个品种的贫血严重程度均呈比例降低。临近分娩时HCT下降，两个品种均降至29%，但产后每天增加0.09个单位，这反映了与寄生虫感染无关的生理适应。在第四次采样时观察到低蛋白血症（6.6 g/dL），与EPG升高同时出现。与贫血母羊（10.19 µg/dL）相比，非贫血母羊（17.84 µg/dL）检测到更高的血清皮质醇水平，尤其是在产后阶段。氧化应激标志物未观察到显著变化。该研究得出结论，低蛋白血症与EPG升高有关。即使最高EPG值为580个虫卵也不表明存在严重的寄生虫感染。在研究过程中，两个品种之间的HCT成比例下降，临近分娩时降至最低值。
