Wardlaw S L, Wang P J, Frantz A G
Life Sci. 1985 Nov 18;37(20):1941-7. doi: 10.1016/0024-3205(85)90012-8.
The effect of estradiol on the brain concentration of immunoreactive beta-endorphin (beta-EP) and C-terminal ACTH (CLIP) was studied in ovariectomized rats. Dopamine, a known inhibitor of pituitary intermediate lobe pro-opiomelanocortin (POMC), was examined as a possible mediator of the estradiol induced changes in brain POMC. Animals were treated for 1 or 3 weeks with either 1) saline; 2) silastic estradiol implants; or 3) estradiol implants plus haloperidol 1 mg/kg/day. After one week of treatment no significant change in hypothalamic beta-EP content was noted in any group compared to the control level of 4.13 +/- .33 (SEM) pmoles although in the neurointermediate lobe beta-EP increased from 566 +/- 72 to 942 +/- 73 pmoles after haloperidol (p less than .005). After 3 weeks, however, hypothalamic beta-EP decreased from 3.96 +/- .28 to 2.74 +/- .19 pmoles (p less than .005) and C-terminal ACTH decreased from 3.78 +/- .33 to 2.82 +/- .18 pmoles (p less than .02) in the estradiol treated rats. This estradiol induced decrease in the hypothalamic content of beta-EP and C-terminal ACTH was not blocked by haloperidol. We conclude that estradiol lowers the hypothalamic content of beta-EP and CLIP and that this effect does not appear to be mediated by dopamine.
在去卵巢大鼠中研究了雌二醇对脑内免疫反应性β-内啡肽（β-EP）和促肾上腺皮质激素C末端（CLIP）浓度的影响。多巴胺是已知的垂体中间叶阿片-促黑素细胞皮质素原（POMC）抑制剂，被作为雌二醇诱导脑内POMC变化的可能介质进行检测。动物用以下方法处理1或3周：1）生理盐水；2）硅橡胶雌二醇植入物；或3）雌二醇植入物加氟哌啶醇1mg/kg/天。治疗1周后，与对照组水平4.13±.33（SEM）皮摩尔相比，任何组的下丘脑β-EP含量均无显著变化，尽管在神经中间叶，氟哌啶醇处理后β-EP从566±72增加到942±73皮摩尔（p<0.005）。然而，3周后，雌二醇处理的大鼠下丘脑β-EP从3.96±.28降至2.74±.19皮摩尔（p<0.005），促肾上腺皮质激素C末端从3.78±.33降至2.82±.18皮摩尔（p<0.02）。雌二醇诱导的下丘脑β-EP和促肾上腺皮质激素C末端含量降低未被氟哌啶醇阻断。我们得出结论，雌二醇降低下丘脑β-EP和CLIP的含量，且这种作用似乎不是由多巴胺介导的。
