Xue Yu-Tao, Tan Ning, Yu Gang-Yan, Tan Li, Lu Yang
Pharmaceutical Department, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
Chin Med Sci J. 2018 Jun 30;33(2):107-113. doi: 10.24920/11806.
Objective To investigate whether red ginseng extract can affect the pharmacokinetics of aspirin in Sprague Dawley (SD) rats.Methods Totally, 12 male SD rats were randomly and uniformly divided into the aspirin group (aspirin 10.42 mg·kg ) and the combined group (red ginseng extraction 0.5 mg·g + aspirin 10.42 mg·kg ). After intragastric administration of drugs, blood samples (0.5 ml once) were drawn from orbit at 0.083, 0.25, 0.5, 1, 2, 4, 6, 8, 10 and 12 hours after dosing. Plasma concentration of salicylic acid (metabolite of aspirin) was detected with ultraviolet-visible high performance liquid chromatography (HPLC). The reliability of the procedure was verified with respect to specificity, linearity, accuracy, precision, extraction recovery and matrix effect, and stability. Pharmacokinetics of salicylic acid was evaluated by using non-compartmental model. Results The method was proved to be validated. Compared with the aspirin group, area under the curve (AUC ) and maximum concentration of salicylic acid in rats of the combined group increased obviously (P<0.01), while clearance rate (CL/F) decreased clearly (P<0.05).Conclusion The in vivo study showed that red ginseng extract can help the internal absorption of aspirin, and delay the in vivo metabolism of aspirin.
目的 研究红参提取物是否会影响阿司匹林在斯普拉格-道利（SD）大鼠体内的药代动力学。方法 将12只雄性SD大鼠随机均分为阿司匹林组（阿司匹林10.42 mg·kg ）和联合组（红参提取物0.5 mg·g + 阿司匹林10.42 mg·kg ）。给药后，于给药后0.083、0.25、0.5、1、2、4、6、8、10和12小时从眼眶取血样（每次0.5 ml）。采用紫外-可见高效液相色谱法（HPLC）检测血浆中水杨酸（阿司匹林代谢物）的浓度。从特异性、线性、准确度、精密度、提取回收率、基质效应和稳定性方面验证该方法的可靠性。采用非房室模型评估水杨酸的药代动力学。结果 该方法经验证有效。与阿司匹林组相比，联合组大鼠水杨酸的曲线下面积（AUC ）和最大浓度明显升高（P<0.01），而清除率（CL/F）明显降低（P<0.05）。结论 体内研究表明，红参提取物可促进阿司匹林的体内吸收，并延缓阿司匹林的体内代谢。
