Kuppers R C, Henney C S
J Immunol. 1977 Jan;118(1):71-6.
The relationship between antigen recognition and lytic expression by killer T cells was studied by co-culturing two effector cell populations. When antigen recognition was bidirectional (e.g., b anti-d cells cultured with d anti-b) there was a loss of lytic activity in both populations. In contrast, when antigen recognition was unidirectional (e.g., a anti-d co-cultured with d anti-b) then the loss of lytic activity only occurred in that direction; i.e., there was a marked decline in the d anti-b activity but no change in the a anti-d population. These studies suggest: i) that mere proximity to a killer cell does not lead to target cell death; ii) that accommodation of the T cell's antigen receptor is necessary for the cell to express its lytic potential; and iii) there is direct linkage between the T cell's antigen receptor site and its killing mechanism.
通过共培养两个效应细胞群体，研究了杀伤性T细胞的抗原识别与裂解表达之间的关系。当抗原识别是双向的（例如，b抗d细胞与d抗b细胞共培养）时，两个群体的裂解活性均丧失。相反，当抗原识别是单向的（例如，a抗d与d抗b共培养）时，裂解活性仅在该方向上丧失；即，d抗b活性显著下降，但a抗d群体没有变化。这些研究表明：i）仅仅靠近杀伤细胞不会导致靶细胞死亡；ii）T细胞抗原受体的适应性对于细胞表达其裂解潜能是必要的；iii）T细胞的抗原受体位点与其杀伤机制之间存在直接联系。
