Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
J Clin Microbiol. 2018 Aug 27;56(9). doi: 10.1128/JCM.00850-18. Print 2018 Sep.
Metagenomics approaches based on shotgun next-generation sequencing hold promise for infectious disease diagnostics. Despite substantial challenges that remain, work done over the past few years justifies excitement about the potential for these approaches to transform how clinical pathogen identification and analysis are performed. In an article in this issue of the , M. I. Ivy et al. (J Clin Microbiol 56:e00402-18, 2018, https://doi.org/10.1128/JCM.00402-18) have applied a shotgun metagenomics approach to the diagnosis of prosthetic joint infections directly from synovial fluid. The results from this work demonstrate both the potentials and challenges of this approach applied in the clinical microbiology laboratory.
基于高通量测序的宏基因组学方法有望应用于传染病的诊断。尽管仍存在重大挑战，但过去几年的工作使人们对这些方法有可能改变临床病原体识别和分析的方式感到兴奋。在本期《临床微生物学杂志》上，M.I. Ivy 等人（J Clin Microbiol 56:e00402-18, 2018, https://doi.org/10.1128/JCM.00402-18）直接从滑液中应用高通量宏基因组学方法诊断人工关节感染。这项工作的结果既展示了该方法在临床微生物学实验室中的潜力，也展示了其面临的挑战。
