一个编码大豆球蛋白A1a亚基前体的cDNA克隆。
A cDNA clone encoding a glycinin A1a subunit precursor of soybean.
作者信息
Negoro T, Momma T, Fukazawa C
出版信息
Nucleic Acids Res. 1985 Sep 25;13(18):6719-31. doi: 10.1093/nar/13.18.6719.
A cDNA clone covering the whole coding region for a glycinin subunit precursor containing the A1a acidic subunit, one of the A2 family, has been identified from a library of soybean cotyledonary cDNA clones using a mixed oligonucleotide probe. Analysis of the cDNA insert revealed that it contained 1746 nucleotides of mRNA sequence with a 5'-terminal nontranslated region of 54 nucleotides, a signal peptide region corresponding to 19 amino acids, an acidic subunit region (A1a) corresponding to 291 amino acids followed by a basic subunit region corresponding to 185 amino acids, and a 3'-terminal nontranslated region of 207 nucleotides. By comparing the predicted protein sequence of this precursor with that of the legumin A precursor of pea, it was found that glycinin A2 subunit family appeared to be more closely related to the legumin than to the A3 subunit family, and that the evolutional rearrangement of glycinin genes has occurred.
利用混合寡核苷酸探针，从大豆子叶cDNA克隆文库中鉴定出一个覆盖含A1a酸性亚基（A2家族之一）的大豆球蛋白亚基前体整个编码区的cDNA克隆。对该cDNA插入片段的分析表明，它含有1746个核苷酸的mRNA序列，5'端非翻译区有54个核苷酸，对应19个氨基酸的信号肽区，对应291个氨基酸的酸性亚基区（A1a），其后是对应185个氨基酸的碱性亚基区，以及3'端207个核苷酸的非翻译区。通过将该前体的预测蛋白质序列与豌豆豆球蛋白A前体的序列进行比较，发现大豆球蛋白A2亚基家族与豆球蛋白的关系似乎比与A3亚基家族更为密切，并且大豆球蛋白基因发生了进化重排。
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