Forde J, Malpica J M, Halford N G, Shewry P R, Anderson O D, Greene F C, Miflin B J
Nucleic Acids Res. 1985 Oct 11;13(19):6817-32. doi: 10.1093/nar/13.19.6817.
A cloned 8.2 kb EcoRI fragment has been isolated from a genomic library of DNA derived from Triticum aestivum L. cv. Cheyenne. This fragment contains sequences related to the high molecular weight (HMW) subunits of glutenin, proteins considered to be important in determining the elastic properties of gluten. The cloned HMW subunit gene appears to be derived from chromosome 1A. The nucleotide sequence of this gene has provided new information on the structure and evolution of the HMW subunits. However, hybrid-selection translation experiments suggest that this gene is silent.
从源自普通小麦品种夏延的DNA基因组文库中分离出了一个克隆的8.2 kb EcoRI片段。该片段包含与麦谷蛋白高分子量(HMW)亚基相关的序列,麦谷蛋白被认为在决定面筋弹性特性方面很重要。克隆的HMW亚基基因似乎源自1A染色体。该基因的核苷酸序列为HMW亚基的结构和进化提供了新信息。然而,杂交选择翻译实验表明该基因是沉默的。