Liu Qian, Zhu Yaxi, Amadio Peter C, Moran Steven L, Gingery Anne, Zhao Chunfeng
Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, USA.
Department of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, China.
Stem Cells Int. 2018 Jun 6;2018:3697971. doi: 10.1155/2018/3697971. eCollection 2018.
Tendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new large animal model for flexor tendon-related research. However, turkey tissue-specific stem cells have not been investigated. Here, we presented the isolation and verification of tendon-derived stem cells (TDSCs) from 6- to 8-month-old heritage-breed turkey. TDSCs were isolated from turkey flexor tendon by plating nucleated cells at the determined optimal density. Approximately 4% of the nucleated cells demonstrated clonogenicity, high proliferation rate, and trilineage differentiation potential after induction culturing. These cells expressed surface antigens CD90, CD105, and CD44, but did not express CD45. There was a high level of gene expression of tenogenic markers in TDSCs, including mohawk, collagen type I, tenascin C, and elastin. Turkey TDSCs also expressed transcription factors , , and , which are critically involved in the regulation of stemness. The successful isolation of tendon-derived stem cells from turkey was beneficial for future studies in tendon tissue engineering and would help in the development of new treatment for tendon diseases using this novel animal model.
肌腱损伤是最常见且严重的手部损伤之一，对功能恢复的需求很高。为了再生医学的目的，已从不同物种和多种组织中鉴定并分离出干细胞。最近，火鸡被提议作为屈肌腱相关研究的潜在新型大型动物模型。然而，火鸡组织特异性干细胞尚未得到研究。在此，我们展示了从6至8月龄传统品种火鸡中分离和验证肌腱衍生干细胞（TDSCs）的过程。通过以确定的最佳密度接种有核细胞，从火鸡屈肌腱中分离出TDSCs。诱导培养后，约4%的有核细胞表现出克隆形成能力、高增殖率和三系分化潜能。这些细胞表达表面抗原CD90、CD105和CD44，但不表达CD45。TDSCs中肌腱生成标记物的基因表达水平很高，包括莫霍克基因、I型胶原蛋白、腱生蛋白C和弹性蛋白。火鸡TDSCs还表达转录因子 、 和 ，它们在干性调节中起关键作用。从火鸡成功分离出肌腱衍生干细胞有利于未来肌腱组织工程研究，并将有助于利用这种新型动物模型开发肌腱疾病的新治疗方法。
