Zhu Fuqiang, Liu Zhendong, Ren Yuxin
Department of Spine Surgery, Dezhou People's Hospital, Dezhou, Shandong 253014, P.R. China.
Department of Orthopedics, Dezhou People's Hospital, Dezhou, Shandong 253014, P.R. China.
Exp Ther Med. 2018 Jul;16(1):192-196. doi: 10.3892/etm.2018.6141. Epub 2018 May 9.
The effects of melatonin and calcium carbonate on aged rats with osteoporosis (OP) were assessed. Forty female Sprague-Dawley (SD) rats aged 15 months were randomly divided into a model group (group OP), melatonin group (group M), calcium carbonate group (group Ca) and melatonin combined with calcium carbonate group (group M+Ca), while 10 rats aged 3 months were set as the control group (group NC). The changes of bone density and bone mineral level of lumbar vertebra and bilateral femur in rats of each group were observed. The levels of serum calcium, phosphorus, superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in rats of each group were determined. Compared with those in group NC, bone density of lumbar vertebra and bilateral femur and bone mineral level were distinctly reduced, serum calcium and activities of SOD and GSH-Px were obviously decreased, and MDA content was remarkably increased in rats of groups OP, M and Ca; the differences were statistically significant (P<0.05 or P<0.01); compared with that in group OP, bone density of lumbar vertebra and bilateral femur and bone mineral level were remarkably increased, serum calcium and activities of SOD and GSH-Px were obviously increased, and MDA content was remarkably decreased in rats of groups M, Ca and M+Ca; the differences were statistically significant (P<0.05 or P<0.01); compared with those in groups M and Ca, bone density of lumbar vertebra and bilateral femur and bone mineral level were obviously elevated, serum calcium and activities of SOD and GSH-Px were evidently elevated, and MDA content was remarkably decreased in rats of group M+Ca; the differences were statistically significant (P<0.05). Melatonin and calcium carbonate can significantly improve antioxidative ability in rats with osteoporosis, increase bone density, elevate serum calcium level and reduce bone mineral loss, thus preventing and treating osteoporosis, and the combination displays more remarkable effects.
评估了褪黑素和碳酸钙对老年骨质疏松（OP）大鼠的影响。将40只15月龄的雌性斯普拉格-道利（SD）大鼠随机分为模型组（OP组）、褪黑素组（M组）、碳酸钙组（Ca组）和褪黑素联合碳酸钙组（M+Ca组），同时将10只3月龄大鼠设为对照组（NC组）。观察各组大鼠腰椎和双侧股骨的骨密度和骨矿物质水平变化。测定各组大鼠血清钙、磷、超氧化物歧化酶（SOD）、丙二醛（MDA）和谷胱甘肽过氧化物酶（GSH-Px）水平。与NC组相比，OP组、M组和Ca组大鼠腰椎和双侧股骨的骨密度和骨矿物质水平明显降低，血清钙以及SOD和GSH-Px活性明显下降，MDA含量显著增加；差异有统计学意义（P<0.05或P<0.01）；与OP组相比，M组、Ca组和M+Ca组大鼠腰椎和双侧股骨的骨密度和骨矿物质水平显著增加，血清钙以及SOD和GSH-Px活性明显增加，MDA含量显著降低；差异有统计学意义（P<0.05或P<0.01）；与M组和Ca组相比，M+Ca组大鼠腰椎和双侧股骨的骨密度和骨矿物质水平明显升高，血清钙以及SOD和GSH-Px活性明显升高，MDA含量显著降低；差异有统计学意义（P<0.05）。褪黑素和碳酸钙可显著提高骨质疏松大鼠的抗氧化能力，增加骨密度，提高血清钙水平，减少骨矿物质流失，从而防治骨质疏松，且联合使用效果更显著。
