Faculty of Medicine, Department of Medical Biochemistry and Molecular Biology, Assiut University, Assiut, 71515, Egypt.
Faculty of Medicine, Department of Histology, Assiut University, Assiut, Egypt.
Appl Biochem Biotechnol. 2023 Aug;195(8):5034-5052. doi: 10.1007/s12010-022-04038-9. Epub 2022 Jul 27.
Postmenopausal osteoporosis is a critical issue for female health worldwide. This current study was designed to evaluate the role of nanopowder eggshell (NPES) in healthy and ovariectomy-induced osteoporosis rats. Fifty-six female rats were divided into healthy rats (35) and ovariectomized rats (21). The healthy rats were subdivided into five groups (G1-G5) and received one of the following treatments: saline, 20 or 40 mg/kg of calcium carbonate, and 20 or 40 mg/kg of NPES. The 21 ovariectomized rats were divided into three groups (G6-G8) and received either saline, 40 mg/kg of calcium carbonate, or 40 mg/kg of NPES. Biochemical and histopathological assessments of bone formation and resorption were performed. Biomarkers of bone formation (calcium and osteocalcin (OCN)) and calcium content in left femur ashes were significantly higher in healthy rats given 40-mg/kg NPES than in healthy control rats and healthy rats given 40-mg/kg calcium carbonate. The ovariectomized groups had significantly lower levels of vitamin D3, OCN, and osteoprotegerin (OPG) than the healthy control. Alanine transaminase (ALT), alkaline phosphatase (ALP), and receptor activator of nuclear factor-κB ligand (RANKL) were significantly increased in the ovariectomized group than in the healthy control group. Treatment with NPES and calcium carbonate reduced liver enzymes in ovariectomized rats. NPES treatment significantly increased Vit D3, OCN, OPG, and bone ash mineral content (calcium, magnesium, zinc, and phosphorus) in ovariectomized rats. NPES also increased femur cortical thickness, osteoblast number, and collagen fiber. The current study suggests that NPES can modulate bone turnover biomarkers and increase bone trace elements. Moreover, NPES alleviates bone resorption in ovariectomy-induced osteoporosis.
绝经后骨质疏松症是全球女性健康的一个重要问题。本研究旨在评估纳米蛋壳粉（NPES）在健康和卵巢切除诱导骨质疏松大鼠中的作用。将 56 只雌性大鼠分为健康大鼠（35 只）和卵巢切除大鼠（21 只）。健康大鼠分为五组（G1-G5），并接受以下治疗之一：生理盐水、20 或 40mg/kg 碳酸钙和 20 或 40mg/kg NPES。21 只卵巢切除大鼠分为三组（G6-G8），并接受生理盐水、40mg/kg 碳酸钙或 40mg/kg NPES。进行骨形成和吸收的生化和组织病理学评估。骨形成的生物标志物（钙和骨钙素（OCN））和左股骨灰分中的钙含量在给予 40mg/kg NPES 的健康大鼠中明显高于健康对照组大鼠和给予 40mg/kg 碳酸钙的健康大鼠。卵巢切除组的维生素 D3、OCN 和骨保护素（OPG）水平明显低于健康对照组。与健康对照组相比，丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和核因子-κB 受体激活剂配体（RANKL）在卵巢切除组中显著增加。NPES 和碳酸钙治疗可降低卵巢切除大鼠的肝酶。NPES 治疗可显著增加卵巢切除大鼠的 Vit D3、OCN、OPG 和骨灰分矿物质含量（钙、镁、锌和磷）。NPES 还增加了股骨皮质厚度、成骨细胞数量和胶原纤维。本研究表明，NPES 可以调节骨转换生物标志物并增加骨微量元素。此外，NPES 可减轻卵巢切除诱导的骨质疏松症中的骨吸收。
