Sawhney Shikhar, Bansal Sandeep, Kalyan Madhur, Verma Indu, Singh Virk Ramandeep, Gupta Ashok Kumar
Department of Otolaryngology, Head and Neck Surgery, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Department of Biochemistry, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Allergy Rhinol (Providence). 2018 Apr 9;9:2152656718764199. doi: 10.1177/2152656718764199. eCollection 2018 Jan-Dec.
Ever since its characterization in the 1970s, allergic fungal rhinosinusitis (AFRS) has been the subject of much controversy, especially regarding its pathogenesis. In this study, we analyzed the differential expression of genes that encode protease-activated receptors (PAR) in patients with AFRS and patients with chronic rhinosinusitis, and tried to understand the pathogenic basis of this disease.
To analyze the differential expression of PAR genes in patients with AFRS and in patients with chronic rhinosinusitis.
Mucosa from ethmoid sinuses of 51 patients (tests and controls) was biopsied and evaluated for messenger RNA expression of PAR genes by using reverse transcriptase-polymerase chain reaction. Each of the four PAR genes, i.e., par1, par2, par3 and par4 was amplified, the final gene products were run on 1.8% agarose gel and analyzed by densitometry to calculate differential expression. The significance level was determined as p ≤ 0.05.
It was observed that the expressions of all four par genes were higher in the test samples compared with the controls, but statistical significance was achieved only for par1 (p=0.004) and par2 (p=0.05). Comparative expression of the four PAR genes was also performed within the test and control groups, and a statistically significant difference was seen between par1 and par2 (p=0.007), par1 and par3 (p=0.029), par1 and par4 (p=0.0001), par2 and par4 (p=0.002), and par3 and par4 (p=0.009) in the test group. In the control group as well, par1, par2, and par3 exhibited a higher expression compared with par4 but the difference was significant between par3 and par4 genes only.
Patients with AFRS expressed increased levels of PAR genes in their nasal mucosa, and, of the four PAR genes, a higher expression of par1, par2, and par3 was observed in both the groups compared with par4. This information contributes toward our understanding of pathogenesis and possibly treatment of AFRS.
自20世纪70年代被描述以来，变应性真菌性鼻-鼻窦炎（AFRS）一直备受争议，尤其是在其发病机制方面。在本研究中，我们分析了AFRS患者和慢性鼻-鼻窦炎患者中编码蛋白酶激活受体（PAR）的基因的差异表达，并试图了解该疾病的发病基础。
分析AFRS患者和慢性鼻-鼻窦炎患者中PAR基因的差异表达。
对51例患者（试验组和对照组）筛窦黏膜进行活检，采用逆转录-聚合酶链反应评估PAR基因的信使核糖核酸表达。对四个PAR基因，即par1、par2、par3和par4分别进行扩增，最终基因产物在1.8%琼脂糖凝胶上进行电泳，并通过光密度测定法进行分析以计算差异表达。显著性水平设定为p≤0.05。
观察到与对照组相比，试验组中所有四个par基因的表达均较高，但仅par1（p=0.004）和par2（p=0.05）具有统计学意义。还在试验组和对照组内对四个PAR基因的表达进行了比较，试验组中par1与par2（p=0.007）、par1与par3（p=0.029）、par1与par4（p=0.0001）、par2与par4（p=0.002）以及par3与par4（p=0.009）之间存在统计学显著差异。在对照组中，par1、par2和par3的表达也高于par4，但仅par3与par4基因之间差异显著。
AFRS患者鼻黏膜中PAR基因表达水平升高，在四个PAR基因中，两组中par1、par2和par3的表达均高于par4。这些信息有助于我们理解AFRS的发病机制并可能为其治疗提供帮助。
