Wood James B, Sesler Cheryl, Stalons Donald, Grigorenko Elena, Schoenecker Jonathan G, Creech C Buddy, Thomsen Isaac P
Division of Pediatric Infectious Diseases and Global Health, Department of Pediatrics, Indianapolis, Indiana.
Division of Pediatric and Adolescent Comparative Effectiveness Research, Indiana University School of Medicine, Indianapolis, Indiana.
Open Forum Infect Dis. 2018 May 22;5(6):ofy119. doi: 10.1093/ofid/ofy119. eCollection 2018 Jun 1.
Improved diagnostics are needed for children with musculoskeletal infections (MSKIs). We assessed the performance of target-enriched multiplex polymerase chain reaction (TEM-PCR) in children with MSKI. TEM-PCR was concordant with culture in pathogen identification and antibiotic susceptibility testing, while increasing the overall yield of pathogen detection. This technology has the potential to inform judicious antimicrobial use early in the disease course.
对于患有肌肉骨骼感染（MSKI）的儿童，需要改进诊断方法。我们评估了靶向富集多重聚合酶链反应（TEM-PCR）在MSKI儿童中的性能。TEM-PCR在病原体鉴定和抗生素敏感性测试方面与培养结果一致，同时提高了病原体检测的总体产量。这项技术有可能在疾病进程早期为明智地使用抗菌药物提供依据。
